Protoplasma

, Volume 194, Issue 3–4, pp 133–139 | Cite as

Immunocytochemical localization of callose in the germinated pollen ofCamellia japonica

  • Y. Hasegawa
  • S. Nakamura
  • N. Nakamura
Article

Summary

A polyclonal antibody against β-1,3-glucan, callose, extracted from the pollen tube wall ofCamellia japonica was raised in mice and, using it as a probe, the localization of callose in the germinated pollen was studied. By confocal laser scanning microscopy, callose was found in the tip region of the pollen tube and the tube wall; the immuno-fluorescence in the tube wall was less toward the base of the tube. In contrast, the tip region did not fluoresce although the whole of the tube wall did strongly with aniline blue, the specific dye for callose. Immuno-electron microscopy showed that callose was also found in Golgi vesicles which concentrated in the tip region of the pollen tube, the inner layer of the tube wall, callose plugs, and Golgi vesicles in the pollen grain. Immuno-gold labeling was often detected on the fibrous structures in Golgi vesicles and callose plugs. Based on these results, the participation of Golgi vesicles in the formation of the tube wall and callose plugs was discussed.

Keywords

Camellia japonica Callose Pollen tube Callose plug Golgi vesicle Immuno-localization 

Abbreviation

TBS

Tris-buffered saline

Tris

Tris(hydroxy-methyl)-aminomethane

PBS

phosphate-buffered saline

BSA

bovine serum albumin

ELISA

enzyme-linked immunosorbent assay

CLSM

confocal laser scanning microscopy

DP

degree of polymerization

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Y. Hasegawa
    • 1
  • S. Nakamura
    • 2
  • N. Nakamura
    • 1
  1. 1.Department of BiologyYokohama City UniversityYokohamaJapan
  2. 2.Biological LaboratoryKanagawa Dental CollegeYokosuka

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