Abstract
The nonhydrolyzable fluorescent diether analog of phosphatidylcholine, 1-O-hexadecyl-2-0-pyrenedecyl-sn-glycero-3-phosphocholine, has been synthesized as a stable probe for the determination of phospholipid transfer to different lipoprotein classes with potential phospholipase activities. After incubation of total human serum with the new probe at 37°C for 3 hours a characteristic partition equilibrium between the main lipoprotein fractions was observed. The fluorescent lipid was not degraded under these conditions and, therefore, served as a marker for choline glycerophospholipid distribution between and transport to serum lipoproteins.
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Gorges, R., Prenner, E., Kostner, G.M. et al. Transfer of pyrene-dietherphosphatidylcholine to serum lipoproteins. J Fluoresc 4, 367–368 (1994). https://doi.org/10.1007/BF01881459
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DOI: https://doi.org/10.1007/BF01881459