Journal of Fluorescence

, Volume 4, Issue 4, pp 353–356 | Cite as

Time-resolved fluorescence anisotropy measurements on fluorescently tagged amphiphilic micelles

  • K. Procházka
  • Z. Limpouchová
  • S. E. Webber
  • P. Munk
Fluorescence of Membranes


Block copolymers of polystyrene-block-poly)methacrylic acid) form multimolecular micelles in mixtures of 1,4-dioxane with a surplus of methanol at ambient temperatures. Micelles consist of compact polystyrene cores surrounded by outer shells formed by poly(methacrylic acid) and are in a reversible equilibrium with nonmicellized copolymer chains (unimers). A series of light scattering, ultracentrifugation, and fluorimetric measurements was performed on micellizing systems of end-tagged copolymers. Complex time-resolved fluorescence anisotropy decays may be explained by a distribution of fluorophores in two microenvironments, i.e., in compact polystyrene micellar cores and in unimer coils.

Key Words

Time-resolved fluorescence anisotropy light scattering amphiphilic block copolymer micelles 


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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • K. Procházka
    • 1
  • Z. Limpouchová
    • 1
  • S. E. Webber
    • 2
  • P. Munk
    • 2
  1. 1.Department of Physical and Macromolecular ChemistryCharles UniversityPrague 2Czech Republic
  2. 2.Department of Chemistry and BiochemistryUniversity of Texas at AustinAustin

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