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Visualization of lipid-receptor interactions on single cells by time-resolved imaging fluorescence microscopy

  • Fluorescence Imaging and Microscopy
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Abstract

The physical interaction between plasma-membrane lipids and the epidermal growth factor (EGF)-receptor was investigated on single A431 human epidermoid carcinoma cells by monitoring fluorescence resonance energy transfer (FRET) between exogeneously added fluorescein-EGF (donor) and 2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphocholine (Bodipy-PC, acceptor) using donor-photobleaching FRET-microscopy. The measured mean FRET-efficiency of 13% is indicative of such a physical interaction and exemplifies the great potential and sensitivity of time-resolved imaging fluorescence microscopy techniques for the study of lipid-receptor interactions on single cells.

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Author notes

  1. T. W. J. Gadella Jr.

    Present address: Department of Biochemistry, Agricultural University of Wageningen, Dreijenlaan 3, NL-6703 HA, Wageningen, The Netherlands

Authors and Affiliations

  1. Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, P.O. Box 2841, D-37018, Göttingen, Germany

    T. W. J. Gadella Jr., D. J. Arndt-Jovin & T. M. Jovin

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  1. T. W. J. Gadella Jr.
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  2. D. J. Arndt-Jovin
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  3. T. M. Jovin
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Gadella, T.W.J., Arndt-Jovin, D.J. & Jovin, T.M. Visualization of lipid-receptor interactions on single cells by time-resolved imaging fluorescence microscopy. J Fluoresc 4, 295–298 (1994). https://doi.org/10.1007/BF01881443

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  • DOI: https://doi.org/10.1007/BF01881443

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