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Comparison of enzyme-cytochemical findings and immunological marker investigations in acute lymphatic leukemia (ALL)

Zytochemische und immunologische Befunde bei akuter lymphatischer Leukämie

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Zusammenfassung

APh und PAS wurden qualitativ und quantitativ bei 50 Fällen von ALL bestimmt, die gleichzeitig durch immunologische Marker-untersuchungen als O- und T-ALL klassifiziert worden waren. Das rein morphologische Bild der Leukämiezellen war nicht mit den Befunden der enzymzytochemischen oder der Membranmarker-Untersuchungen korreliert. Die Durchschnittswerte APh-positiver Zellen und eines errechneten Index lagen bei T-ALL signifikant höher als bei O-ALL, für PAS-anfärbbares Material verhielten sich die Ergebnisse umgekehrt: höhere Werte bei O-ALL, niedrigere bei T-ALL. Für beide Reaktionen wurden aber Ausnahmen beobachtet. Zytochemische Befunde können also einen wichtigen Beitrag für die weitere Unterteilungen der ALL leisten, sollten aber möglichst durch Membranmarker-Untersuchungen ergänzt werden.

Summary

APh-activity and PAS-positive deposits were studied in 50 cases of ALL, classified as T-ALL and O-ALL according to immunological marker investigations. Correlation between morphological features of the cells and APh and PAS reactions, as well as between morphology and immunological markers was not detected. APh-activity in general was stronger in T-ALL (R+ and R−), while PAS-content was more pronounced in O-ALL. The results suggest that cytochemical methods, especially APh and PAS reaction, are valuable to distinguish T-ALL from O-ALL but not reliable enough to replace immunological marker investigations.

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Abbreviations

ALL:

acutelymphoblastic leukemia

APh:

acid phosphatase

FITC-anti-Ig:

fluorescein isothiocyanate labelled anti human immunoglobulin

O-ALL:

non-T, non-B=“null” acute lymphoblastic leukemia

PAS:

periodic acid-Schiff's reaction

Pox:

peroxidase

SmIg:

surface membrane immunoglobulin

SRBC:

Sheep red blood cells

T-ALL:

T-cell acute lymphoblastic leukemia

T-ALL (R+):

T-ALL, E-rosettes positive

T-ALL (R-):

T-ALL, E-rosettes negative

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Authors and Affiliations

  1. Medical Clinic III, Großhadern, University of Munich, Klinikum Großhadern, Marchioninistraße 15, D-8000, Munich 70, Federal Republic of Germany

    P. Andreewa &  D. Huhn

  2. Institute for Haematology and Blood Transfusion Medical Academy, Sofia, Bulgaria

    P. Andreewa

  3. Dept. of Immunology, Inst. Haematology, GSF, Munich, D-8000, Munich, Federal Republic of Germany

    E. Thiel & H. Rodt

Authors
  1. P. Andreewa
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  2. D. Huhn
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  3. E. Thiel
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  4. H. Rodt
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Andreewa, P., Huhn, D., Thiel, E. et al. Comparison of enzyme-cytochemical findings and immunological marker investigations in acute lymphatic leukemia (ALL). Blut 36, 299–305 (1978). https://doi.org/10.1007/BF01880680

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