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, Volume 36, Issue 5, pp 263–273 | Cite as

Demonstration of the Fc-receptor of blood cells by soluble peroxidase-anti-peroxidase (PAP) complexes

  • D. Huhn
  • P. Andreewa
  • H. Rodt
  • E. Thiel
  • M. Eulitz
Original Papers

Summary

The Fc-receptor of normal human leukocytes, of CLL-cells, and of hematopoietic cell lines was demonstrated with soluble peroxidase-anti-peroxidase (PAP) complexes. In about 9% of normal lymphocytes an almost continuous, strong labeling of the cell membrane was established. Some of these lymphocytes were characterized by a peculiar uniform fine structure. The percentage of PAP-labeled monocytes was in the range of 25%, neutrophils nearly 100%, eosinophils 0%, CLL-cells 10%. Labeled portions of the membrane were interiorized from monocytes. The lymphoid cell-line Daudi established from a Burkitt's lymphoma appeared almost negative, the cell line K 562 established from a myeloid leukemia in 75% of the cells strongly positive. PAP-labeling was not influenced by preincubation with trypsine or with neuraminidase; it was negative when PAP-F(ab)2 was used. Results of PAP-labeling were not always in agreement with EA-rosettes or with agg-Ig.

Key words

Membrane markers Peroxidase-immunochemistry Fc-receptor 

Abbreviations

Agg-Ig

heat-aggregated human immunoglobulin (for demonstration of Fc-receptor)

ATG

anti-T-cell immunglobulin

BSA

bovine serum albumin

CLL

chronic lymphatic leukemia

EA-rosettes

rosettes with human erythrocytes sensitized by IgG (for demonstration of Fc-receptor)

Ig

immunoglobulin

PAP

peroxidase-conjugated anti-peroxidase-antibody

PBS

phosphate-buffered saline

POX

peroxidase

Nachweis des Fc-Rezeptors von Blutzellen durch lösliche Peroxidase-anti-Peroxidase (PAP)-Komplexe

Zusammenfassung

Der Fc-Rezeptor von normalen menschlichen Leukozyten, CLL-Zellen und hämatopoetischen Zell-Linien wurde mittels löslicher Peroxidase-anti-Peroxidase (PAP)-Komplexe untersucht. Etwa 9% normaler Lymphozyten zeigten eine starke, nahezu ununterbrochene Membranmarkierung. Einige dieser stark markierten Lymphozyten waren durch eine gemeinsame, auffällige Feinstruktur gekennzeichnet. PAP-positive Monozyten lagen in der Größenordnung von 25%, Neutrophile fast 100%, Eosinophile 0%, CLL-Zellen 10%. Markierte Membrananteile wurden von Monozyten ins Zellinnere aufgenommen. Die lymphoide Zell-Linie Daudi war kaum mit PAP zu markieren, die myeloide Zell-Linie K 562 war zu 75% stark positiv. Die PAP-Markierung war durch Vorinkubation mit Trypsin oder Neuraminidase nicht zu beeinflussen; Kontrollen mit PAP-F(ab)2 blieben vollständig negativ. Die Ergebnisse der PAP-Markierung stimmten mit EA-Rosetten und agg-Ig nicht immer überein.

Schlüsselwörter

Membranmarkeirung Peroxidase Immunchemic Fc-Rezeptor 

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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • D. Huhn
    • 1
  • P. Andreewa
    • 1
    • 2
  • H. Rodt
    • 3
  • E. Thiel
    • 3
  • M. Eulitz
    • 3
  1. 1.Dept. of Medicine III, GroßhadernUniv of MunichMunich 70Federal Republic of Germany
  2. 2.Inst. for Haematology and Blood TransfusionMedical AcademySofiaBulgaria
  3. 3.Dept. of Immunology of the Inst. of HematologyGSF, MunichMunich 2Federal Republic of Germany

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