Skip to main content
SpringerLink
Log in

Demonstration of the Fc-receptor of blood cells by soluble peroxidase-anti-peroxidase (PAP) complexes

Nachweis des Fc-Rezeptors von Blutzellen durch lösliche Peroxidase-anti-Peroxidase (PAP)-Komplexe

  • Original Papers
  • Published:
Blut Aims and scope Submit manuscript

Zusammenfassung

Der Fc-Rezeptor von normalen menschlichen Leukozyten, CLL-Zellen und hämatopoetischen Zell-Linien wurde mittels löslicher Peroxidase-anti-Peroxidase (PAP)-Komplexe untersucht. Etwa 9% normaler Lymphozyten zeigten eine starke, nahezu ununterbrochene Membranmarkierung. Einige dieser stark markierten Lymphozyten waren durch eine gemeinsame, auffällige Feinstruktur gekennzeichnet. PAP-positive Monozyten lagen in der Größenordnung von 25%, Neutrophile fast 100%, Eosinophile 0%, CLL-Zellen 10%. Markierte Membrananteile wurden von Monozyten ins Zellinnere aufgenommen. Die lymphoide Zell-Linie Daudi war kaum mit PAP zu markieren, die myeloide Zell-Linie K 562 war zu 75% stark positiv. Die PAP-Markierung war durch Vorinkubation mit Trypsin oder Neuraminidase nicht zu beeinflussen; Kontrollen mit PAP-F(ab)2 blieben vollständig negativ. Die Ergebnisse der PAP-Markierung stimmten mit EA-Rosetten und agg-Ig nicht immer überein.

Summary

The Fc-receptor of normal human leukocytes, of CLL-cells, and of hematopoietic cell lines was demonstrated with soluble peroxidase-anti-peroxidase (PAP) complexes. In about 9% of normal lymphocytes an almost continuous, strong labeling of the cell membrane was established. Some of these lymphocytes were characterized by a peculiar uniform fine structure. The percentage of PAP-labeled monocytes was in the range of 25%, neutrophils nearly 100%, eosinophils 0%, CLL-cells 10%. Labeled portions of the membrane were interiorized from monocytes. The lymphoid cell-line Daudi established from a Burkitt's lymphoma appeared almost negative, the cell line K 562 established from a myeloid leukemia in 75% of the cells strongly positive. PAP-labeling was not influenced by preincubation with trypsine or with neuraminidase; it was negative when PAP-F(ab)2 was used. Results of PAP-labeling were not always in agreement with EA-rosettes or with agg-Ig.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

Agg-Ig:

heat-aggregated human immunoglobulin (for demonstration of Fc-receptor)

ATG:

anti-T-cell immunglobulin

BSA:

bovine serum albumin

CLL:

chronic lymphatic leukemia

EA-rosettes:

rosettes with human erythrocytes sensitized by IgG (for demonstration of Fc-receptor)

Ig:

immunoglobulin

PAP:

peroxidase-conjugated anti-peroxidase-antibody

PBS:

phosphate-buffered saline

POX:

peroxidase

References

  1. Abramson, N., Gelfand, E.W., Jandl, J.H., Rosen, F.S.: The interaction between human monocytes and red cells. Specificity for IgG subclasses and IgG fragments. J. exp. Med.132, 1207–1215 (1970)

    PubMed  Google Scholar 

  2. Basten, A., Miller, J.F.A.P., Sprent, J., Pye, J.: A receptor for antibody on B lymphocytes. I. Method of detection and functional significance. J. exp. Med.125, 610–626 (1972)

    Google Scholar 

  3. Basten, A., Miller, J.F.A.P., Abraham, R.: Relationship between Fc receptors, antigenbinding sites on T and B cells, and H-2 complex-associated determinants. J. exp. Med.141, 547–560 (1975)

    PubMed  Google Scholar 

  4. Cossmann, J., Schnitzer, B., Deegan, M.J.: Immunologic surface markers in non-Hodgkin's lymphomas. Amer. J. Path.1, 19–27 (1977)

    Google Scholar 

  5. Dickler, H.B., Kunkel, H.G.: Interaction of aggregatedγ-globulin with B lymphocytes. J. exp. Med.136, 191–196 (1972)

    PubMed  Google Scholar 

  6. Dickler, H.B.: Studies of the human lymphocyte receptor for heat aggregated or antigen-complexed immunoglobulin. J. exp. Med.140, 508–522 (1974)

    PubMed  Google Scholar 

  7. Dickler, H.B.: Lymphocyte binding of aggregated immunoglobulin. Scand. J. Immunol. Suppl.5, 91–97 (1976)

    Google Scholar 

  8. Documenta Geigy. Wissenschaftliche Tabellen, pp. 170, 177, 1960

  9. Douglas, S.D., Huber, H.: Electron microscopic studies of human monocyte and lymphocyte interaction with immunoglobulin- and complement-coated erythrocytes. Exp. Cell Res.70, 161–172 (1972)

    PubMed  Google Scholar 

  10. Ferrarini, M., Tonda, G.P., Risso, A., Viale, G.: Lymphocyte membrane receptors in human lymphoid leukemias. Europ. J. Immunol.5, 89–93 (1975)

    Google Scholar 

  11. Frieß, A.E., Liebich, H.-G.: Ultrahistochemische Untersuchungen an der Glykokalyx von Lymphozyten aus dem Ductus thoracicus der Ratte. Z. Zellforsch.134, 143–152 (1972)

    PubMed  Google Scholar 

  12. Frøland, S.S., Natvig, J.B.: Identification of three different human lymphocyte populations by surface markers. Transplant. Rev.16, 114–162 (1973)

    PubMed  Google Scholar 

  13. Frøland, S.S., Natvig, J.B., Michaelsen, T.E.: Binding of aggregated IgG by human B lymphocytes independent of Fc receptors. Scand. J. Immunol.3, 375–380 (1974)

    PubMed  Google Scholar 

  14. Huber, H., Fudenberg, H.: Receptor sites of human monocytes for IgG. Int. Arch. Allergy34, 18 (1968)

    PubMed  Google Scholar 

  15. Huber, H., Douglas, S.D., Nusbacher, J., Kochwa, S., Rosenfield, R.E.: IgG subclass specificity of human monocyte receptor sites. Nature229, 419–420 (1971)

    PubMed  Google Scholar 

  16. Huber, Ch., Sundström, C., Nilsson, K., Wigzell, H.: Surface receptors on human haematopoietic cell lines. Clin. exp. Immunol.25, 367–376 (1976)

    PubMed  Google Scholar 

  17. Huhn, D.: Neue Organelle im peripheren Lymphozyten? Dtsch. med. Wschr.93, 2099–2100 (1968)

    PubMed  Google Scholar 

  18. Jondal, M., Klein, G.: Surface markers on human B and T lymphocytes. II. Presence of Epstein-Barr virus receptors on B lymphocytes. J. exp. Med.138, 1365–1378 (1973)

    PubMed  Google Scholar 

  19. Karnovsky, M.J.: The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J. Cell Biol.35, 213–236 (1967)

    PubMed  Google Scholar 

  20. Klein, E., Klein, G., Nadkarni, J. S., Nadkarni, J.J., Wigzell, H., Clifford, P.: Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res.28, 1300–1310 (1968)

    PubMed  Google Scholar 

  21. Koziner, B., McKenzie, S., Straus, D., Clarkson, B., Good, R.A., Siegal, F.P.: Cell marker analysis in acute monocytic leukemias. Blood49, 895–901 (1977)

    PubMed  Google Scholar 

  22. Lobo, P.I., Horwitz, D.A.: An appraisal of Fc receptors on human peripheral blood B and L lymphocytes. J. Immunol.117, 939–943 (1976)

    PubMed  Google Scholar 

  23. Lozzio, C.B., Lozzio, B.B.: Human chronic myelogenous leukemia cell line with positive Philadelphia chromosome. Blood45, 321–334 (1975)

    PubMed  Google Scholar 

  24. McKeever, P.E., Garvin, A.J., Spicer, S.S.: Immune complex receptors on cell surfaces. I. Ultrastructural demonstration on macrophages. J. Histochem. Cytochem.24, 948–955 (1976)

    PubMed  Google Scholar 

  25. McKeever, P.E., Garvin, A.J., Hardin, D.H., Spicer, S.S.: Immune complex receptors on cell surfaces. II. Cytochemical evaluation of their abundance on different immune cells: Distribution, uptake, and regeneration. Amer. J. Path.84, 437–449 (1976)

    PubMed  Google Scholar 

  26. Messner, R.P., Jelinek, J.: Receptors for human γG-globulin on human neutrophils. J. clin. Invest.49, 2165–2171 (1970)

    PubMed  Google Scholar 

  27. Nisonoff, A., Wissler, F.C., Lipman, L.N., Woernley, D.L.: Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch. Biochem. Biophys.89, 230–244 (1960)

    PubMed  Google Scholar 

  28. Pichler, W.J., Knapp, W.: Chronic lymphatic leukemia cells bear IgM receptors. In: Immunological Diagnosis of Leukemias and Lymphomas. Thierfelder, S., Rodt, H., Thiel, E. (eds.) pp. 163–168. Berlin, Heidelberg, New York: Springer 1977

    Google Scholar 

  29. Schmidt, M.E., Douglas, S.D.: Disappearance and recovery of human monocyte IgG receptor activity after phagocytosis. J. Immunol.109, 914–917 (1972)

    PubMed  Google Scholar 

  30. Steinman, R.M., Cohn, Z.A.: The interaction of soluble horseradish peroxidase with mouse peritoneal macrophages in vitro. J. Cell Biol.55, 186–204 (1972)

    PubMed  Google Scholar 

  31. Steinman, R.M., Cohn, Z.A.: The interaction of particulate horseradish peroxidase (HRP)-anti HRP immune complexes with mouse peritoneal macrophages in vitro. J. Cell Biol.55, 616–634 (1972)

    PubMed  Google Scholar 

  32. Sternberger, L.A., Hardy Jr., P.H., Cuculis, J.J., Meyer, H.G.: The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigenantibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J. Histochem. Cytochem.18, 315–333 (1970)

    PubMed  Google Scholar 

  33. Thiel, E., Dörmer, P., Rodt, H., Huhn, D., Bauchinger, M., Kley, H.P., Thierfelder, S.: Quantitation of T-antigenic sites and Ig-determinants on leukemic cells by microphotometric immunoautoradiography. Proof of the clonal origin of thymus-derived lymphocytic leukemias. In: Immunological Diagnosis of Leukemias and Lymphomas. Thierfelder, S., Rodt, H., Thiel, E. (eds.), pp. 131–145. Berlin, Heidelberg, New York: Springer 1977

    Google Scholar 

  34. Unanue, E.R., Cerottini, J.-C.: The function of macrophages in the immune reponse. Sem. Hemat.7, 225–248 (1970)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept. of Medicine III, Großhadern, Univ of Munich, Marchioninistraße 15, D-8000, Munich 70, Federal Republic of Germany

    D. Huhn & P. Andreewa

  2. Inst. for Haematology and Blood Transfusion, Medical Academy, Sofia, Bulgaria

    P. Andreewa

  3. Dept. of Immunology of the Inst. of Hematology, GSF, Munich, Landwehrstraße 61, D-8000, Munich 2, Federal Republic of Germany

    H. Rodt, E. Thiel & M. Eulitz

Authors
  1. D. Huhn
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Andreewa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Rodt
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Thiel
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Eulitz
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Huhn, D., Andreewa, P., Rodt, H. et al. Demonstration of the Fc-receptor of blood cells by soluble peroxidase-anti-peroxidase (PAP) complexes. Blut 36, 263–273 (1978). https://doi.org/10.1007/BF01880677

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01880677

Schlüsselwörter

Key words

Search

Navigation