Scopulariopsis brevicaulis: Effect of pH and substrate on growth

  • R. J. Bothast
  • E. B. Lancaster
  • C. W. Hesseltine
Food Microbiology


Scopulariopsis brevicaulis Bainier NRRL 5867, isolated from ammonia-treated corn during preservation studies, was grown in shaken and still liquid cultures on Blakeslee's malt extract. The medium was adjusted to different pH values between 5.0 and 10.6 with sodium hydroxide and ammonium hydroxide. Maximum mycelium was produced at an initial pH of 9.0–10.0. Considerably more mycelium was produced in shaken flasks than in still cultures. When the initial pH was adjusted to 10.0 with ammonium hydroxide, 1350 mg mycelium/200 ml Blakeslee's malt extract was produced in contrast to 540 mg with sodium hydroxide. Approximately 28% of the total solids and 25% of the nitrogen in an ammoniated corn infusion broth were converted to mold mycelium high in essential amino acids and protein by both NRRL 5867 and NRRL 3273, another strain ofS. brevicaulis. WhenS. brevicaulis was grown 7 days on a solid substrate of ammoniated corn, ammonia was converted to organic material, carbohydrate was utilized and the protein of the fermented corn increased in lysine and methionine. Approximately 9% of the weight of the corn was lost during the process.


Ammonium Corn Carbohydrate Hydroxide Mold 
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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • R. J. Bothast
    • 1
  • E. B. Lancaster
    • 1
  • C. W. Hesseltine
    • 1
  1. 1.Northern Regional Research Laboratory, Agricultural Research ServiceU.S. Department of AgriculturePeoria

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