Summary
Scopulariopsis brevicaulis Bainier NRRL 5867, isolated from ammonia-treated corn during preservation studies, was grown in shaken and still liquid cultures on Blakeslee's malt extract. The medium was adjusted to different pH values between 5.0 and 10.6 with sodium hydroxide and ammonium hydroxide. Maximum mycelium was produced at an initial pH of 9.0–10.0. Considerably more mycelium was produced in shaken flasks than in still cultures. When the initial pH was adjusted to 10.0 with ammonium hydroxide, 1350 mg mycelium/200 ml Blakeslee's malt extract was produced in contrast to 540 mg with sodium hydroxide. Approximately 28% of the total solids and 25% of the nitrogen in an ammoniated corn infusion broth were converted to mold mycelium high in essential amino acids and protein by both NRRL 5867 and NRRL 3273, another strain ofS. brevicaulis. WhenS. brevicaulis was grown 7 days on a solid substrate of ammoniated corn, ammonia was converted to organic material, carbohydrate was utilized and the protein of the fermented corn increased in lysine and methionine. Approximately 9% of the weight of the corn was lost during the process.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
AOAC. (1970). Official methods of analysis. 11th ed. Association of Official Analytical Chemists, Washington, D.C.
Bainier, G. (1970a). Bull. Soc. Mycol. Fr. 23, 98–105
Bainier, G. (1970b). Bull. Soc. Mycol. Fr. 23, 125–127
Bothast, R.J., Adams, G.H., Hatfield, E.E., Lancaster, E.B. (in press) J. Dairy Sci.
Bothast, R.J., Lancaster, E.B., Hesseltine, C.W. (1973). J. Dairy Sci. 56, 241–245
Christensen, C.M., Nelson, G.H., Mirocha, C.J., Bates, F. (1968). Cancer Res. 28, 2293–2295
Dennis, C., Gee, J.M. (1973). J. Gen. Microbiol. 78, 101–107
Frazier, W.C. (1958). Food microbiology. 2nd ed. McGraw-Hill Book Company, New York
Halbrook, E.R., Winter, A.R., Sutton, T.S. (1951). Poultry Sci. 30, 381–388
Lancaster, E.B., Hall, G.E., Brekke, O.L. (1974). Trans. ASAE 17, 331–338
Lorah, M.E., Funk, E.M., Forward, J. (1954). Mo. Agr. Exp. Sta. Res. Bull. 573
Lovett, J. (1972). Poultry Sci. 51, 309–313
Lovett, J., Messer, J.W., Read, R.B. (1971). Poultry Sci. 50, 746–751
Martin, P.M.D., Gilman, G.A., Keen, P. (1971). The incidence of fungi in foodstuffs and their significance based on a survey in the Eastern Transvaal and Swaziland. In: Symposium on Mycotoxins in Human Health, pp. 281–289, Ed. I.F.H. Purchase. The Macmillan Co., London
Morton, A.G., Broadbent, D. (1955). J. Gen. Microbiol. 12, 248–258
Morton, A.G., MacMillan, A. (1954). J. Exp. Bot. 5, 232–252
Morton, F.J., Smith, G. (1963). Mycological Papers, No. 86
Pike, R., Brown, M.L. (1967). Nutrition: An integrated approach. John Wiley & Sons, Inc.
Raper, K.B., Thom, C. (1949). A manual of the penicillia. Bailliere, Tindall & Cox, London
Rhodes, R.A., Hall, H.H., Anderson, R.F., Nelson, G.E.N., Shekleton, M.C., Jackson, R.W. (1961). Appl. Microbiol. 9, 181–184
Schefferle, H.E. (1965). J. Appl. Bacteriol. 28, 403–411
Thom, C. (1930). The penicillia. Bailliere, Tindall & Cox, London
Thom, C., Raper, K.B. (1932). Science 76, 548–550
Uhl, D.E., Lancaster, E.B., Vojnovich, C. (1971). Anal. Chem. 43, 990–994
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bothast, R.J., Lancaster, E.B. & Hesseltine, C.W. Scopulariopsis brevicaulis: Effect of pH and substrate on growth. European J. Appl Microbiol. 1, 55–66 (1975). https://doi.org/10.1007/BF01880620
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01880620