An improved method for assessing soil-fungus population density
- 302 Downloads
A rapid method for determining soil fungal populations is described. A 25-g equivalent of oven-dry soil, in 225 ml of water in a 500-ml aspirator bottle, is agitated on a magnetic stirrer and samples withdrawn with a syringe while the suspension is in motion. Individual drops are placed in petri dishes and mixed with a suitable culture medium. The average weight of soil per drop is determined and colony counts related to dry weight of soil as usual. This syringe method is simple, eliminates much of the error common in serial dilution and pipetting, and allows for a high degree of reproducibility and possible standardization.
KeywordsSyringe Plant Physiology Population Density Average Weight Petri Dish
Unable to display preview. Download preview PDF.
- 1.Brierley, W. B., Jewson, S. T. and Brierley, M., The quantitative study of soil fungi. Proc. First Intern. Congr. Soil Sci.3, 48–71 (1927).Google Scholar
- 2.Duncan, D. B., Multiple range and multiple F tests. Biometrics11, 1–42 (1955).Google Scholar
- 3.Hornby, D. and Ullstrup, A. J., Physical problems of sampling soil suspensions in the dilution-plate technique. Phytopathology55, 1062 (1965) (Abstr.).Google Scholar
- 4.James, N. and Sutherland, M., The accuracy of the plating method for estimating the numbers of soil bacteria, actinomycetes and fungi in the dilution plate. Can. J. Research C17, 72–86 (1939).Google Scholar
- 5.Johnson, L. F., Curl, E. A., Bond, J. H., and Fribourg, H. A., Methods for Studying Soil Microflora-Plant Disease Relationships. Burgess Publishing Co., Minneapolis, Minn. (1959).Google Scholar
- 6.Rodriguez-Kabana, R. and Funderburk, Jr., H. H., A method for assessing fungal density in herbicide-treated soils. Phytopathology55, 1073–1074 (1965) (Abstr.).Google Scholar
- 7.Schenck, N. C. and Curl, E. A., A short quantitative method for estimating the population density of soil fungi. Proc. Soil Crop Sci. Soc. Florida21, 13–17 (1961).Google Scholar
- 8.Warcup, J. H., The soil-plate method for isolation of fungi from soil. Nature166, 117–118 (1950).Google Scholar