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Immunologische Grundlagen der allergischen Spätreaktion

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Zusammenfassung

An transferaktiven Zellen von tb-sensibilisierten Tieren sind mindestens zwei distinkte zellständige Antikörper nachweisbar. Eine Art dieser Antikörper ist gegen Tuberkulopolysaccharid und die andere gegen Tuberkuloprotein gerichtet. Sie haben beide nach Kontakt mit dem Antigen die Fähigkeit, Komplement zu binden. Eine Cytolyse kommt sowohl durch eine zellständig ablaufende Immunreaktion zwischen Polysaccharid-Antikörper und Tuberkuloprotein-Antikörper mit dem Antigen zustande, jedoch ist dazu nur im letzteren Falle Komplement erforderlich. Nach Kontakt mit dem Antigen ist also beim Polysacharid-Antikörper eine Komplementbindung möglich, für eine Cytolyse aber nicht nötig. Die beiden zellständigen Antikörper lassen sich also nach ihrer Antigenspezifität und im Hinblick auf den Mechanismus ihres zellschädigenden Effektes unterscheiden.

Summary

Cellular antibodies of at least two specificities can be demonstrated on peritoneal lymphocytes of tb-sensitized animals. One specificity is directed to the polysaccharide fraction of tuberculin whereas the other specificity refers to the protein fraction of tuberculin. Both antibodies can be demonstrated on cells by their capacity to fix successively tuberculin material and complement; both of these reactions can be detected by immunofluorescent staining. The interaction of antibody containing cells with tuberculoproteins and also with tuberculopolysaccharides results in cytolysis. The protein cytolysis is dependent on the presence of complement whereas the polysaccharide-lysis is independent of it. Both antibodies can be obtained in soluble form when cells are extracted. The soluble form of the cellular antibodies can be transferred to normal lymphocytes.

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  1. Aus dem Institut für Medizinische Mikrobiologie der Universität Mainz, Germany

    G. Gillissen

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Nach einem Vortrag auf dem Symposium der Schweizerischen Gesellsachaft für Allergieforschung, Davos, 2. April 1964.

Die hier vorgelegten eigenen Untersuchungen wurden durch Mittel der Deutschen Forschungsgemeinschaft und der Robert Koch-Stiftung unterstützt.

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Gillissen, G. Immunologische Grundlagen der allergischen Spätreaktion. Klin Wochenschr 43, 590–597 (1965). https://doi.org/10.1007/BF01879179

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