Abstract
The effects of high excitation intensities in fluorescence correlation spectroscopy (FCS) in terms of saturation and triplet-state build-up have been studied for the case of Rh6G in aqueous solution. It was found that FCS provides a powerful means for the determination of intersystem crossing and triplet-state depopulation rates of fluorophores in solution.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
E. L. Elson and D. Madge (1974)Biopolymers 13, 1–27.
M. Ehrenberg and R. Rigler (1974)Chem. Phys. 4, 390–401.
R. Rigler, J. Widengren, and Ü Mets (1992) in O. S. Wolfbeis (Ed.)Fluorescence Spectroscopy, Springer-Verlag, Berlin/Heidelberg/New York, pp. 13–24.
R. Rigler Ü, Mets, J. Widengren, and P. Kask (1993).Eur. Biophys. J. 22, 169–175.
R. Rigler and Ü. Mets (1992) inLaser Spectroscopy of Biomolecules, SPIE Vol. 1921, pp. 239–248.
V. E. Korobov and A. K. Chibisov (1978)J. Photochem. 9, 411–424.
R. P. Haugland (1992).Handbook of Fluorescent Probes and Research Chemicals, Molecular Probes, Inc., Eugene, Oregon, p. 218.
Author information
Authors and Affiliations
Additional information
This is a peer-reviewed conference proceeding article from the Third Conference on Methods and Applications of Fluorescence Spectroscopy, Prague, Czech Republic, October 18–21, 1993.
Rights and permissions
About this article
Cite this article
Widengren, J., Rigler, R. & Mets, Ü. Triplet-state monitoring by fluorescence correlation spectroscopy. J Fluoresc 4, 255–258 (1994). https://doi.org/10.1007/BF01878460
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01878460