Journal of Fluorescence

, Volume 4, Issue 3, pp 247–250 | Cite as

lin-Benzo-ATP and-ADP: Versatile fluorescent probes for spectroscopic and biochemical studies

  • E. Grell
  • E. Lewitzki
  • C. Bremer
  • S. Kramer-Schmitt
  • J. Weber
  • A. E. Senior
Regular Articles
  • 49 Downloads

Abstract

lin-Benzo-adenine nucleotides can act not only as probes for fluorescence studies but also as structural active site probes for enzymes. To understand the basic properties oflin-benzo-ATP and-ADP, protolysis and Mg2+ and Ca2+, binding are investigated between pH 6.2 and pH 8.5 by spectrophotometric and spectrofluorometric titrations. Based on a reaction model, a set of equilibrium constants is determined which is consistent with all available experimental results. The pK values of the Mg2+ and Ca2+ complex oflin-benzo-ATP in the chosen medium are 4.6 and 4.1, respectively, and those for the corresponding diphosphate are 3.1 and 2.8, respectively. Fluorescence and absorption spectra are reported.

Key Words

lin-Benzo-ATP lin-benzo-ADP dissociation constants Mg2+ and Ca2+ binding fluorescence spectra 

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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • E. Grell
    • 1
  • E. Lewitzki
    • 1
  • C. Bremer
    • 1
  • S. Kramer-Schmitt
    • 1
  • J. Weber
    • 2
  • A. E. Senior
    • 2
  1. 1.Max-Planck-Institute of BiophysicsFrankfurtGermany
  2. 2.Department of BiochemistryUniversity of Rochester Medical CenterRochester

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