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A tumor cell line producing granulocyte colony-stimulating factor and an immune suppressive factor

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Biotherapy

Abstract

From a patient, both a cell line incapable of secreting granulocyte colony-stimulating factor (G-CSF) (TC873) and a cell line capable of secreting G-CSF (TCM902) were established. The effector cells induced, with TC873 cells showed a high lytic capacity against two types of tumor cells. The effector cells induced by TCM902 cells did not show such capacity. Furthermore, the TCM902 cells excreted a factor suppressing the proliferation of lymphokine activated killer (LAK) cells and the autologous tumor cell lysis of tumor associated lymphocytes. This factor probably is TFG-β 1.

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Abbreviations

CSF:

colony stimulating factor

ELISA:

enzyme-linked immunosorbent assay

G:

granulocyte

GM:

granulocyte-monocyte

IFN:

interferon

IL:

interleukin

LAK:

lymphokine activated killer

M:

monocyte

MLTC:

mixed lymphocyte tumor cell culture

TGF:

transforming growth factor

TILs:

tumor infiltrating lymphocytes

TNF:

tumor necrosis factor

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Authors and Affiliations

  1. Department of Otorhinolaryngology, Yokohama City University, School of Medicine, Fukuura 3-9, Kanazawa-ku, Yokohama, 236, Kanagawa, Japan

    Mamoru Tsukuda, Taro Nagahara, Yasukazu Mikami, Tadayuki Yago & Hideki Matsuda

  2. Department of Biochemistry, Kanagawa Cancer Center, Nakao-cho 54-6, Asahi-ku, Yokohama, 241, Kanagawa, Japan

    Shunsuke Yanoma

Authors
  1. Mamoru Tsukuda
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  2. Taro Nagahara
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  3. Yasukazu Mikami
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  4. Tadayuki Yago
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  5. Hideki Matsuda
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  6. Shunsuke Yanoma
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Tsukuda, M., Nagahara, T., Mikami, Y. et al. A tumor cell line producing granulocyte colony-stimulating factor and an immune suppressive factor. Biotherapy 6, 217–223 (1993). https://doi.org/10.1007/BF01878083

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  • DOI: https://doi.org/10.1007/BF01878083

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