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The synergistic effects of interleukin 2 and interleukin 7 on the proliferation and autologous tumor cell lysis of tumor-associated lymphocytes

  • Published:
Biotherapy

Abstract

Interleukin-7 (IL-7) has an ability to stimulate the proliferation of pre-B cells. It has been shown that IL-7 can also activate T lymphocytes. We here demonstrate that IL-7 in combination with interleukin-2 (IL-2) can drive cell proliferation and enhance the autologous tumor cell lysis by peripheral blood mononuclear cells (PBMC) and autologous mixed lymphocyte tumor cell culture (MLTC)-derived effector cells (MLTC cells). These synergistic effects of IL-2 and IL-7 on the proliferation and the augmentation of autologous tumor cell lysis were found for both effector cells. These effects were inhibited by neutralizing antibodies to IL-2 or IL-7, and by a combination of both antibodies, significantly. In terms of phenotypical expression, CD3 positive cells comprised the vast majority of MLTC cells after culture in medium containing IL-2 and IL-7 with an increase of IL-2 receptor positive cells.

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Abbreviations

CD:

cluster differentiation

IFN:

interferon

IL:

interleukin

JRU:

Japanese Reference Unit

LAK:

lymphokine activated killer

mAb:

monoclonal antibody

MLTC:

mixed lymphocyte tumor cell culture

PBMC:

peripheral blood mononuclear cells

TILs:

tumor infiltrating lymphocytes

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Authors and Affiliations

  1. Department of Otorhinolaryngology, School of Medicine, Yokohama City University, Fukuura 3-9, Kanazawa-ku, Yokohama, 236, Kanagawa, Japan

    Mamoru Tsukuda, Izumi Mochimatsu, Miki Sakumoto, Yasukazu Mikami & Seiichiro Yuyama

  2. Department of Biochemistry, Kanagawa Cancer Center, Nakao-cho 54-6, Asahi-ku, Yokohama, 241, Kanagawa, Japan

    Shunsuke Yanoma

Authors
  1. Mamoru Tsukuda
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  2. Izumi Mochimatsu
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  3. Miki Sakumoto
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  4. Yasukazu Mikami
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  5. Seiichiro Yuyama
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  6. Shunsuke Yanoma
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Tsukuda, M., Mochimatsu, I., Sakumoto, M. et al. The synergistic effects of interleukin 2 and interleukin 7 on the proliferation and autologous tumor cell lysis of tumor-associated lymphocytes. Biotherapy 6, 167–174 (1993). https://doi.org/10.1007/BF01878077

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  • DOI: https://doi.org/10.1007/BF01878077

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