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Molecular characterization of potato virus YN isolates by PCR-RFLP

Differentiation of PVYN isolates by PCR-RFLP

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Abstract

Based on the sequence polymorphism in the 5′ terminal part of the viral genome, a range of PVYN isolates were characterized by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP). Three pairs of primers selected in the 5′ non-translated and P1 protein region were tested. Two of them yielded PCR products of about 1Kb from all isolates tested. Restriction analysis of the PCR products gave two distinct electrophoretic patterns, whichever of the three enzymes was used. In this way, the 18 isolates were separated into two easily identifiable subgroups. All tuber necrosing isolates (PVYNTN) were clustered in the same subgroup.

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Authors and Affiliations

  1. Station de Pathologie Végétale, INRA, Domaine de La Motte, BP 29, 35650, Rennes-Le Rheu, France

    L. Glais, C. Kerlan & M. Tribodet

  2. Station de Pathologie Végétale, INRA, Route de Saint-Cyr, 78026, Versailles, France

    V. Marie -Jeanne Tordo, C. Robaglia & S. Astier-Manifacier

Authors
  1. L. Glais
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  2. C. Kerlan
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  3. M. Tribodet
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  4. V. Marie -Jeanne Tordo
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  5. C. Robaglia
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  6. S. Astier-Manifacier
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Glais, L., Kerlan, C., Tribodet, M. et al. Molecular characterization of potato virus YN isolates by PCR-RFLP. Eur J Plant Pathol 102, 655–662 (1996). https://doi.org/10.1007/BF01877246

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  • DOI: https://doi.org/10.1007/BF01877246

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