Biotechnology Techniques

, Volume 2, Issue 2, pp 133–136 | Cite as

Occlusion immobilization of hybridoma cells in chitosan

  • E. Wang
  • S. E. Overgaard
  • J. M. Scharer
  • N. C. Bols
  • M. Moo-Young
Article

Summary

The use of chitosan fibers as a matrix for immobilizing hybridoma cells was investigated. Optimal cell entrapment within fibrous chitosan occurred at pH values below 7.2. Chitosan fibers were found to be more effective than chitosan beads in the occlusion of the hybridoma cells. Maximum total cell concentrations of 5 to 5.8 × 106 cells/mL were obtained in the chitosan fractions of cultures containing 25% to 50% chitosan volume fractions. The viability of the cells was found to be unaffected by the presence of chitosan in culture.

Keywords

Chitosan Immobilization Total Cell Cell Concentration Optimal Cell 

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Copyright information

© Science & Technology Letters 1988

Authors and Affiliations

  • E. Wang
    • 1
  • S. E. Overgaard
    • 1
  • J. M. Scharer
    • 1
  • N. C. Bols
    • 1
  • M. Moo-Young
    • 1
  1. 1.Industrial Biotechnology CentreUniversity of WaterlooWaterlooCanada

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