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Estimation of fungal growth on lignocellulosic substrates using an enzyme-linked immunosorbent assay

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Biotechnology Techniques

Summary

The feasibility of using an enzyme-linked immunosorbent assay (ELISA) for quantifying the growth ofThielavia terrestris on cellulosic substrates was studied. WhenT. terrestris was grown, using glucose as the substrate, the ELISA readings correlated well with the mycelial dry weight values. When lignocellulosic substrates were used, the ELISA appeared to slightly overestimate fungal growth. The ELISA values indicated that the fungi grew more slowly on steam-treated aspenwood than on commercial sources of cellulose (Avicel).

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Authors and Affiliations

  1. Biotechnology and Chemistry Department, Forintek Canada Corp., 800 Montreal Road, K1G 3Z5, Ottawa, Ontario

    C. Breuil, G. Wojtczak & J. N. Saddler

  2. Department of Biology, University of Ottawa, Ottawa, Ontario

    D. J. Kushner

Authors
  1. C. Breuil
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  2. G. Wojtczak
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  3. D. J. Kushner
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  4. J. N. Saddler
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Breuil, C., Wojtczak, G., Kushner, D.J. et al. Estimation of fungal growth on lignocellulosic substrates using an enzyme-linked immunosorbent assay. Biotechnol Tech 3, 247–252 (1989). https://doi.org/10.1007/BF01876058

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  • DOI: https://doi.org/10.1007/BF01876058

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