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Measurement of ammonia and glutamine in cell culture media by gas sensing electrodes

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Biotechnology Techniques

Summary

This paper describes the use of a commercially available off-line gas sensing electrode for determination of ammonia and glutamine in cell culture media. The measurement technique was tested in different media preparations with different serum concentrations. The glutamine decomposition was studied as a function of pH for cell culture medium and the results were compared to those obtained by conventional methods,i.e., HPLC. Finally, glutamine and ammonia metabolism were studied during the cultivation of a hybridoma cell line.

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Authors and Affiliations

  1. Department of Chemistry, The University of Michigan, 48109, Ann Arbor, MI, USA

    Mark E. Meyerhoff

  2. Department of Chemical Engineering, The University of Michigan, 48109, Ann Arbor, MI, USA

    Sadettin S. Ozturk & Bernhard O. Palsson

Authors
  1. Sadettin S. Ozturk
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  2. Mark E. Meyerhoff
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  3. Bernhard O. Palsson
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Ozturk, S.S., Meyerhoff, M.E. & Palsson, B.O. Measurement of ammonia and glutamine in cell culture media by gas sensing electrodes. Biotechnol Tech 3, 217–222 (1989). https://doi.org/10.1007/BF01876051

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  • DOI: https://doi.org/10.1007/BF01876051

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