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Use of piezoelectric shock waves to effect release of amylase from capsules containing the amylase producing rat pancreatic tumour cell line AR42J

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Biotechnology Techniques

Summary

The amylase-producing rat pancreatic tumour cell line AR42J was encapsulated in alginate/poly-L-lysine under conditions where the secreted amylase was retained within the system. On treatment with shock waves from a lithotriptor, the porosity of the capsules was changed and the amylase was released from the system. Conditions of treatment were manipulated in order to maximise release of the amylase without significantly reducing the cell viability.

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Authors and Affiliations

  1. Dept. of Microbiology, Moyne Institute, Trinity College, Dublin 2, Ireland

    U. McCarthy, L. Bunni, J. Seary & A. P. McHale

  2. Meath Hospital, Heytesbury St., Dublin 8, Ireland

    B. Jones & M. Butler

  3. National Institute for Higher Education, Glasnevin, Dublin 9, Ireland

    L. McHale

Authors
  1. B. Jones
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  2. U. McCarthy
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  3. L. Bunni
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  4. L. McHale
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  5. M. Butler
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  6. J. Seary
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  7. A. P. McHale
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Jones, B., McCarthy, U., Bunni, L. et al. Use of piezoelectric shock waves to effect release of amylase from capsules containing the amylase producing rat pancreatic tumour cell line AR42J. Biotechnol Tech 3, 355–360 (1989). https://doi.org/10.1007/BF01875636

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  • DOI: https://doi.org/10.1007/BF01875636

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