Skip to main content
SpringerLink
Log in

Micro isoelectric point heterogeneity of a murine monoclonal antibody (L6) originating from cell cultivation conditions

  • Published:
Biotechnology Techniques

Summary

Isoelectric point (pI) microheterogeneity of an IgG monoclonal antibody (L6) was studied by rapid affinity chromatography, isoelectric focusing on polyacrylamide gels, and rapid ion-exchange chromatography. An abiotic or strictly chemical basis for observed changes is postulated. The described methods are useful for monoclonal antibody process monitoring since they are rapid, automated assay procedures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Awdeh, Z.L., Williamson, A.R., and Askonas, B.A. (1970). Biochem. J.116, 241–248.

    Google Scholar 

  • Bouman, H., Meincke, G., and Havsteen, B. (1975). Z. Immun.-Forsch. Bd.150, 370–377.

    Google Scholar 

  • Compton, B.J., Lewis, M.A., Whigham, F., Gerald, J.S., and Countryman, G.E. (1989). Accepted for publication in Anal. Chem., July 1, 1989.

  • Davies, M.T. (1959).84, 248–251.

  • Hamilton, R.G., Reimer, C.B., and Rodkey, L.S. (1987). Hybridoma6(2), 205–217.

    Google Scholar 

  • Nose, M., and Wigzell, H. (1983). Proc. Natl. Acad. Sci. USA80, 6632–6636.

    Google Scholar 

  • Pearson, T.W., and Anderson, N.L. (1983). Methods in Enzymology92, 196–220.

    Google Scholar 

  • Schlaeger, E.J., Eggimann, B., and Gast, A. (1987). Develop. biol. Standard.,66, 403–408.

    Google Scholar 

  • Williamson, A.R., Salaman, M.R., and Kreth, H.W. (1973) Ann. N.Y. Acad. Sci.209, 210–224.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bristol-Myers Company Industrial Division, Syracuse, New York

    B. J. Compton, D. A. Lowe & R. P. Elander

  2. John Hopkins Medical School, Baltimore, Maryland

    J. S. Gerald

Authors
  1. B. J. Compton
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. S. Gerald
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. A. Lowe
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. P. Elander
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Compton, B.J., Gerald, J.S., Lowe, D.A. et al. Micro isoelectric point heterogeneity of a murine monoclonal antibody (L6) originating from cell cultivation conditions. Biotechnol Tech 3, 349–354 (1989). https://doi.org/10.1007/BF01875635

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01875635

Keywords

Search

Navigation