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A method for fractionation of cloned protein in recombinantSaccharomyces cerevisiae

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Biotechnology Techniques

Abstract

In a spheroplasting method which allows the fractionation and quantification of cloned invertase activity in recombinantSaccharomyces cerevisiae cells, the yeast cell is selectively degraded with the enzyme Zymolyase for 60 minutes at 45°C to separate periplasmic proteins from cytoplasmic proteins. Most of the glucose-6-phosphate dehydrogenase (a cytoplasmic marker protein) was found in the cytoplasmic fraction.

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Authors and Affiliations

  1. School of Chemical Engineering, Purdue University, 47907, West Lafayette, Indiana

    Mark Marten & Jin-Ho Seo

Authors
  1. Mark Marten
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  2. Jin-Ho Seo
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Marten, M., Seo, JH. A method for fractionation of cloned protein in recombinantSaccharomyces cerevisiae . Biotechnol Tech 3, 325–328 (1989). https://doi.org/10.1007/BF01875630

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  • DOI: https://doi.org/10.1007/BF01875630

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