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A pH-tyrosinase biosensor for aminoacids, catecholamines and adrenergic drugs determination

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Biotechnology Techniques

Summary

A sensor based on immobilized tyrosinase and a pH electrode has been constructed. The calibration curve of the sensor is linear up to 0.03–0.24 mg/ml depending on the substrate used. The sensor retains 50% of its sensitivity after 10–11 hours, showing 6–7 hours of stationary activity in continuous working. DOPA, tyrosine, isoproterenol, α-methyl-DOPA, DOPAmine, adrenaline and noradrenaline were used as substrates.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Faculty of Chemistry, University of Murcia, Spain

    J. J. Berenguer, A. Manjon & J. L. Iborra

Authors
  1. J. J. Berenguer
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  2. A. Manjon
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  3. J. L. Iborra
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Berenguer, J.J., Manjon, A. & Iborra, J.L. A pH-tyrosinase biosensor for aminoacids, catecholamines and adrenergic drugs determination. Biotechnol Tech 3, 211–216 (1989). https://doi.org/10.1007/BF01875622

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  • DOI: https://doi.org/10.1007/BF01875622

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