Fish Physiology and Biochemistry

, Volume 15, Issue 5, pp 363–370 | Cite as

Effect of environmental calcium levels on calcium uptake in tilapia larvae Oreochromis mossambicus

  • Pung-Pung Hwang
  • Yu-Chi Tung
  • Min-Hwang Chang
Article

Abstract

Effects of environmental calcium concentrations on the survival, growth, body calcium content and calcium uptake kinetics in developing tilapia (Oreochromis mossambicus) larvae were studied. Fertilized eggs were incubated in high- and low-calcium artificial freshwater (0.88–0.96 mmol l−1vs. 0.02–0.03 mmol l−1 CaCl2 or CaSO4) until 3 days after hatching. Tilapia larvae showed similar hatching rates and wet weights in either high- or low-calcium medium, indicating neither the development nor the growth in tilapia larvae was affected by the environmental calcium levels. The body calcium content in low-calcium groups was about 90–95% that of high-calcium groups, No matter what calcium source was used (CaCl2 or CaSO4), acclimation to low calcium medium caused a stimulation of calcium uptake (measured in 0.2 mmol l−1 calcium),i.e., 1.2–1.3 fold higher than that of high calcium groups. This enhanced calcium uptake capacity was characterized by a 50% decrease in Km and a 25% increase in Jmax. Effect of different calcium salts on calcium influx was significant only in low calcium level,i.e., calcium influx in low-CaCl2 group higher than that in low-CaSO4 group. These results suggest that tilapia larvae are able to modulate their calcium uptake mechanism to maintain normal body calcium content and growth in environments with different levels of calcium.

keywords

tilapia larvae development Ca2+ content Ca2+ influx Km, Jmax 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References cited

  1. Alderdice, D.F. 1988. Osmotic and ionic regulation in teleost eggs and larvae.In Fish Physiology. Vol. XI, part A, pp. 163–251. Edited by W.S. Hoar and D.J. Randall. Academic Press, San Diego.Google Scholar
  2. Avella, M., Masoni, A., Bornancin, M. and Mayer-Gostan, N. 1987. Gill morphology and sodium influx in the rainbow trout (Salmo gairdneri) acclimated to artificial freshwater environments. J. Exp. Zool. 241: 159–169.Google Scholar
  3. Ayson, F.G., Kaneko, T., Hasegawa, S. and Hirano, T. 1994. Development of mitochondria-rich cells in the yolk-sac membrane of embryos and larvae of tilapia,Oreochromis mossambicus, in freshwater and seawater. J. Exp. Zool. 270: 129–135.Google Scholar
  4. Flik, G., Fenwick, J.C., Kolar, Z., Mayer-Gostan, N. and Wendelaar Bonga, S.E. 1985. Whole-body calcium flux in cichlid teleost fishOreochromis mossambicus adapted to freshwater. Am. J. Physiol. 249: R432–437.Google Scholar
  5. Flik, G., Fenwick, J.C., Kolar, Z., Mayer-Gostan, N. and Wendelaar Bonga, S.E. 1986. Effects of low ambient calcium levels on whole-body Ca2+ flux rates and internal calcium pools in the freshwater cichlid teleost,Oreochromis mossambicus. J. Exp. Biol. 120: 249–264.Google Scholar
  6. Flik, G., Van Der Velden, J.A., Dechering, K.J., Verbost, P.M., Schoenmakers, T.J.M., Kolar, Z.I. and Wendelaar Bonga, B.E. 1993. Ca2+ and Mg2+ transport in gills and gut of tilapia,Oreochromis mossambicus: a review. J. Exp. Zool. 265: 356–365.Google Scholar
  7. Goss, G.G. and Wood, C.M. 1990. Na+ and Cl uptake kinetics diffusive effluxes and acidic equivalent fluxes across the gills of rainbow trout. I. Response to environmental hyperoxia. J. Exp. Biol. 152: 521–547.Google Scholar
  8. Hwang, P.P. 1990. Salinity effects on development of chloride cells in the larvae of ayu,Plecoglossus altivelis. Mar. Biol. 107: 1–7.Google Scholar
  9. Hwang, P.P. and Hirano, R. 1985. Effects of environmental salinity on intercellular organization and junctional structure of chloride cells in early stages of teleost development. J. Exp. Zool. 236: 115–126.Google Scholar
  10. Hwang, P.P., Tsai, Y.N. and Tung, Y.C. 1994. Calcium balance in embryos and larvae of the freshwater-adapted teleost,Oreochromis mossambicus. Fish Physiol. Biochem. 13: 325–333.Google Scholar
  11. Hwang, P.P. and Wu, S.M. 1993. Role of cortisol in hypoosmoregulation in larvae of the tilapia (Oreochromis mossambicus). Gen. Comp. Endocrinol. 92: 318–324.Google Scholar
  12. Hwang, P.P., Wu, S.M., Lin, J.H. and Wu, L.S. 1992. Cortisol in eggs and larvae of teleosts. Gen. Comp. Endocrinol. 86: 189–196.Google Scholar
  13. Kersetter, T.H. and Kirschner, L.B. 1972. Active chloride transport by the gills of rainbow trout (Salmo gairdneri). J. Exp. Biol. 56: 263–272.Google Scholar
  14. Lauren, D. and MacDonald, D.G. 1987. Acclimation to copper by rainbow trout,Salmo gairdneri: physiology. Can. J. Fish. Aquat. Sci. 43: 1488–1496.Google Scholar
  15. Marshall, W.S., Bryson, S.E. and Wood, C.M. 1992. Calcium transport by isolated skin of rainbow trout. J. Exp. Biol. 166: 297–316.Google Scholar
  16. Mayer-Gostan, N., Bornancin, M., DeRenzis, G., Naon, R., Yee, J.A., Shew, R.L. and Pang, P.K.T. 1983. Extraintestinal calcium uptake in the killifish,Fundulus heteroclitus. J. Exp. Zool. 227: 329–338.Google Scholar
  17. McCormick, S.D., Hasegawa, S. and Hirano, T. 1992. Calcium uptake in the skin of a freshwater teleost. Proc. Nat. Acad. Sci. U.S.A. 89: 3635–3638.Google Scholar
  18. McWilliams, P.G. 1993. Environmental induction of Na+ transporter affinity in Atlantic salmon embryos. J. Fish Biol. 42: 119–130.Google Scholar
  19. McWilliams, P.G. and Shephard, K.L. 1989. Kinetic characteristics of the sodium uptake mechanism during the development of embryos and fry of Atlantic salmon,Salmo salar L., in an improved water quality. J. Fish Biol. 35: 855–868.Google Scholar
  20. Michal, G. 1976. Determination of Michaelis constants and inhibitor constants.In Methods of Enzymatic Analysis. Vol. 1, pp. 144–156. Edited by H.U. Bergmeyer Academic Press, New York.Google Scholar
  21. Payan, P., Mayer-Gostan, N. and Pang, P.K.T. 1981. Site of calcium uptake in the freshwater trout gill. J. Exp. Zool. 216: 345–347.Google Scholar
  22. Perry, S.F. and Flik, G. 1988. Characterization of branchial transepithelial calcium fluxes in freshwater trout,Salmo gairdneri. Am. J. Physiol. 254: R491–498.Google Scholar
  23. Perry, S.F., Goss, G.G. and Fenwick, J.C. 1922. Interrelationships between gill chloride cell morphology and calcium uptake in freshwater teleosts. Fish Physiol. Biochem. 10: 327–337.Google Scholar
  24. Perry, S.F. and Wood, C.M. 1985. Kinetics of branchial calcium uptake in the rainbow trout: effects of acclimation to various external calcium levels. J. Exp. Biol. 116: 411–433.Google Scholar
  25. Schoenmakers, T.J.M., Visser, G.J., Flik, G. and Theuvenet, A.P.R. 1992. Chelator: an improved method for computing metal ion concentration in physiological solutions. Biotechniques 12: 870–879.Google Scholar

Copyright information

© Kugler Publication 1996

Authors and Affiliations

  • Pung-Pung Hwang
    • 1
    • 2
  • Yu-Chi Tung
    • 1
  • Min-Hwang Chang
    • 3
  1. 1.Institute of ZoologyAcademia SinicaTaipeiTaiwan, Republic of China
  2. 2.Institute of Fisheries ScienceNational Taiwan UniversityTaipei
  3. 3.Department of BiologyTunghai UniversityTaichungTaiwan, ROC

Personalised recommendations