A procedure for the measurement of fungal and bacterial α-amylase in crude culture filtrates and commercial enzyme preparations is described. The procedure employs end-blocked (non-reducing end)p-nitrophenyl maltoheptaoside in the presence of amyloglucosidase and α-glucosidase, and is absolutely specific for α-amylase. The assay procedure is simple, reliable and accurate.
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American Association of Cereal Chemists (1987). Approved Methods of the AACC. 8th ed. Method 20-01, approved May 1960. The Association, St. Paul, MN.
Barnes, W.B. and Blakeney, A.B. (1974). Starch. 26, 193–197
Bernfeld, P., (1940). Methods Enzymol. (Colowick, S.P. and Kaplan, N.O. Eds.), 1, 149–158.
Blair, H.E. (1987). U.S. Patent No. 4649108.
Farrand, E.A. (1964). Cereal Chem. 41, 98–111.
Kaufman, R.A. and Tietz, N.W. (1980). Clin. Chem. 26, 846–853.
McCleary, B.V. and Sheehan, H. (1987). J. Cereal Sci. 6, 237–251.
Sandstedt, R.M., Kneen, E. and Blish, M.J. (1939). Cereal Chem. 16, 712–723.
Somogyi, M. (1952). J. Biol. Chem. 195, 19–23.
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Sheehan, H., McCleary, B.V. A new procedure for the measurement of fungal and bacterial α-amylase. Biotechnol Tech 2, 289–292 (1988). https://doi.org/10.1007/BF01875544
- Enzyme Preparation
- Culture Filtrate
- Assay Procedure
- Commercial Enzyme