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A new procedure for the measurement of fungal and bacterial α-amylase

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Biotechnology Techniques

Summary

A procedure for the measurement of fungal and bacterial α-amylase in crude culture filtrates and commercial enzyme preparations is described. The procedure employs end-blocked (non-reducing end)p-nitrophenyl maltoheptaoside in the presence of amyloglucosidase and α-glucosidase, and is absolutely specific for α-amylase. The assay procedure is simple, reliable and accurate.

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Authors and Affiliations

  1. Biocon Biochemicals Ltd., Carrigaline, Co., Cork, Ireland

    Helen Sheehan

  2. Biological and Chemical Research Institute, New South Wales Agriculture and Fisheries, PMB 10, 2116, Rydalmere, NSW, Australia

    Barry V. McCleary

Authors
  1. Helen Sheehan
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  2. Barry V. McCleary
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Sheehan, H., McCleary, B.V. A new procedure for the measurement of fungal and bacterial α-amylase. Biotechnol Tech 2, 289–292 (1988). https://doi.org/10.1007/BF01875544

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  • DOI: https://doi.org/10.1007/BF01875544

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