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A low-cost aqueous two phase system for enzyme extraction

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Biotechnology Techniques


Several low-cost maltodextrins form two liquid phases with polyethylene glycol (PEG) in aqueous solution. We have measured physical properties for three PEG-maltodextrin two-phase systems and demonstrated their bioseparation capabilities by extracting alcohol dehydrogenase from yeast extracts with a PEG bound textile dye.

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Szlag, D.C., Guiliano, K.A. A low-cost aqueous two phase system for enzyme extraction. Biotechnol Tech 2, 277–282 (1988).

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