Skip to main content
SpringerLink
Log in

A refocused and optimized HNCA: Increased sensitivity and resolution in large macromolecules

  • Short Communications
  • Published:
Journal of Biomolecular NMR Aims and scope Submit manuscript

Summary

A 3D optimized, refocused HNCA experiment is described. It is demonstrated to yield a dramatic increase in sensitivity when applied to [13C,15N]-labeled human carbonic anhydrase II, a 29-kDa protein. The reasons for the gain in sensitivity are discussed, and 3 distinct areas for further development are indicated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Bax, A. and Ikura, M. (1991)J. Biomol. NMR,1, 99–104.

    Google Scholar 

  • Bax, A., Ikura, M., Kay, L.E., Torchia, D.A. and Tschudin, R. (1990)J. Magn. Reson.,86, 304–318.

    Google Scholar 

  • Brown, L.R. and Bremer, J. (1986)J. Magn. Reson.,68, 217–231.

    Google Scholar 

  • Bystrov, V.F. (1976)Prog. NMR Spectrosc.,10, 41–81.

    Google Scholar 

  • Campbell-Burk, S., Domaille, P. and Müller, L. (1991)J. Magn. Reson.,93, 171–177.

    Google Scholar 

  • Farmer II, B.T. (1991)J. Magn. Reson.,94, 413–418.

    Google Scholar 

  • Farmer II, B.T.,J. Magn. Reson., submitted for publication.

  • Ikura, M., Kay, L.E. and Bax, A. (1990)Biochemistry,29, 4659–4667.

    Google Scholar 

  • Kay, L.E., Ikura, M., Tschudin, R. and Bax, A. (1990)J. Magn. Reson.,89, 496–514.

    Google Scholar 

  • Levitt, M.H. (1986)Prog. NMR Spectrosc.,18, 61–122.

    Google Scholar 

  • Marion, D., Ikura, M., Tschudin, R. and Bax, A. (1989)J. Magn. Reson.,85, 393–399.

    Google Scholar 

  • Powers, R., Gronenborn, A.M., Clore, G.M. and Bax, A. (1991)J. Magn. Reson.,94, 209–213.

    Google Scholar 

  • Shaka, A.J., Barker, P. and Freeman, R. (1985)J. Magn. Reson.,64, 547–552.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Research and Development, NMR Instruments, Varian Associates, 3120 Hansen Way, 94304, Palo Alto, CA, USA

    B. T. Farmer II

  2. Department of Biochemistry, Duke University Medical Center, 27710, Durham, NC, USA

    R. A. Venters & L. D. Spicer

  3. Bristol Myers Squibb, Pharmaceutical Research Institute, P.O. Box 4000, 08543-4000, Princeton, NJ, USA

    M. G. Wittekind & L. Müller

Authors
  1. B. T. Farmer II
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. A. Venters
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. D. Spicer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. G. Wittekind
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. Müller
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Farmer, B.T., Venters, R.A., Spicer, L.D. et al. A refocused and optimized HNCA: Increased sensitivity and resolution in large macromolecules. J Biomol NMR 2, 195–202 (1992). https://doi.org/10.1007/BF01875530

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01875530

Keywords

Search

Navigation