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Preparation and evaluation of an enzyme which degrades yeast cell walls

  • Reisuke Kobayashi
  • Tan Miwa
  • Shinpei Yamamoto
  • Susumu Nagasaki
Article

Summary

A method for the production and preparation of an enzyme which degrades yeast cell walls from a species of aRhizoctonia (tentatively identified asR. solani) was established on a commercial scale. The production of crude enzyme powder, having a lytic activity of 100 units/mg, in batches of 80 kg is feasible.

The enzyme preparation was evaluated for industrial use. When yeast cells were treated with this enzyme, the digestibility of feed yeast was improved 1.4–2 fold in vitro; the efficiency of a mechanical disintegrator in extracting cellular substances was increased 35–50%; the release of soluble glucans having widely varying degrees of polymerization was induced; the extraction of cellular protein by alkali was facilitated 2–3 fold; an 80% release of cell-bound invertase was induced and 2–3 times more yeast extract could be prepared.

Keywords

Enzyme Polymerization Cell Wall Yeast Extract Yeast Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Reisuke Kobayashi
    • 1
  • Tan Miwa
    • 1
  • Shinpei Yamamoto
    • 2
  • Susumu Nagasaki
    • 2
  1. 1.Chemical Research LaboratoryKumiai Chemical Industry Co., Ltd.ShizuokaJapan
  2. 2.Department of Agricultural Chemistry, Faculty of AgricultureKochi UniversityKochiJapan

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