Abstract
The cellular mechanism of action of 3,5,3′-triiodo-L-thyronine (T3) in enhancing SG-G100 gonadotropin-induced ovarian secretion of 17β-estradiol (E2) was studiedin vitro using oocyte follicular preparations of rainbow trout. The dependence of the T3 stimulatory action on the level of intracellular 3′,5′-cyclic adenosine monophosphate (cAMP) was shown in experiments in which forskolin or dibutyryl cAMP enhanced E2 secretion. In the presence of partially purified salmon gonadotropin (SG-G100), T3 stimulation of E2 secretion was prevented by theophylline, suggesting that T3 may exert part of its stimulatory action by inhibiting phosphodiesterase.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References cited
Cyr, D.G., Duston, J., Bromage, N.R. and Eales, J.G. 1988. Seasonal correlations between serum levels of thyroid hormones and sex steroids in rainbow trout held under various photoperiod regimes. Gen. Comp. Endocrinol. 69: 217–225.
Cyr, D.G. and Eales, J.G. 1988a.In vitro effects of thyroid, hormones on gonadotropin-induced estradiol-17β secretion by ovarian follicles of rainbow trout,Salmo gairdneri. Gen. Comp. Endocrinol. 69: 80–87.
Cyr, D.G. and Eales, J.G. 1988b. The influence, of thyroidal status on ovarian function in rainbow trout,Salmo gairdneri. J. Exp. Zool. 248: 81–87.
Hurlburt, M.E. 1977. Role of thyroid gland in ovarian maturation of goldfish,Carassius auratus L. Can. J. Zool. 55: 1906–1913.
Idler, D.R., Hwang, S.J. and Bazar, L.S. 1975. Fish gonadotropin(s). I. Bioassay of salmon gonadotropin(s),in vitro with immature trout gonads. Endocr. Res. Commun. 2: 199–213.
Rao, G.S., Lemoch, H. and Conrath, C. 1987. Interaction of calmodulin with iodothyronines: Effect of iodothyronines on the calmodulin activation of cyclic AMP phosphodiesterase. Molec. Cell. Endocrinol. 53: 45–52.
Salmon, C., Marchelidon, J., Fontaine-Bertrand, E. and Fontaine, Y.A. 1985. Human chorionic gonadotrophin and immature fish ovary: Characterization and mechanism of action of thein vitro stimulation of cyclic adenosine monophosphate accumulation. Gen. Comp. Endocrinol. 58: 101–108.
Segal, J. and Ingbar, S.H. 1986. Extranuclear receptors for thyroid hormones.In Thyroid Hormone Metabolism. pp. 417–440. Edited by G. Henneman. Marcel Dekkar Inc. New York.
Smoake, J.A., Okmen, A.A., Moy, M. and Solomon, S.S. 1988. Isozymes of plasma membrane phosphodiesterase: The effects of altered thyroid states. Clin. Res. 36: 43A.
Tallarida, R.J. and Murray, R.B. 1981. Manual of Pharmacologic Calculations with Computer Programs. Springer-Verlag, New York.
Tan, J.D., Adachi, S. and Nagahama, Y. 1986. Thein vitro effects of cyclic nucleotides, cyanoketone, and cycloheximide on the production of estradiol-17β by vitellogenic ovarian follicles of goldfish (Carassius auratus). Gen. Comp. Endocrinol. 63: 110–116.
Wasserman, W.J. and Houle, J.G. 1984. The regulation of ribosomal-protein S-6 phosphorylation inXenopus oocytes: A role for intracellular pH. Devel. Biol. 101: 436–445.
Wells, J.N. and Kramer, G.L. 1981. Phosphodiesterase inhibitors as tools in cyclic nucleotide research: A precautionary comment. Mol. Cell. Endocrinol. 23: 1–9.
Zar, J.H. 1974. Biostatistical Analysis. Prentice Hall Inc., Englewood Cliffs.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cyr, D.G., Eales, J.G. T3 enhancement ofin vitro estradiol-17β secretion by oocytes of rainbow trout,Salmo gairdneri, is dependent on cAMP. Fish Physiol Biochem 6, 255–259 (1989). https://doi.org/10.1007/BF01875028
Issue Date:
DOI: https://doi.org/10.1007/BF01875028