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Catecholestrogens inhibit dopamine methylation in the gonadotrops of the African catfish,Clarias gariepinus

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Abstract

Isolated gonadotrops of the African catfish,Clarias gariepinus, were incubated with dopamine (DA) and/or catecholestrone and the activity of the enzyme catechol-O-methyltransferase (COMT) was determined by measuring the methylated products. From the apparent Km values for DA and catecholestrone of 0.4–1.3 μM and 17.9–25.2 μM respectively, it was concluded that catecholestrone is a better substrate for the enzyme COMT, compared to DA. Moreover, the methylation of DA is inhibited by comparatively low concentrations of catecholestrone.

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References cited

  • Ball, P. and Knuppen, R. 1980. Catecholestrogens (2-and 4-hydroxyestrogens) chemistry, biogenesis, metabolism, occurence and physiological significance. Acta Endocrinol. (Copenh.) 93, suppl. 232: 1–128.

    Google Scholar 

  • Billard, R. 1978. Testicular feedback on the hypothalamopituitary axis in rainbow trout (Salmo gairdneri R.). Ann. Biol. Anim. Biochim. Biophys. 18: 813–818.

    Google Scholar 

  • Billard, R., Alagarswami, K., Peter, R.E. and Breton, B. 1983. Potentialisation par le pimozide des effects du LHRH-A sur la sécrétion gonadotrope hypophysaire l'ovulation et la spermiation chez la carpe commune (Cyprinus carpio). C. R. Acad. Sci., Paris 296C: 181–184.

    Google Scholar 

  • Billard, R. and Peter, R.E. 1977. Gonadotropin release after implantation of anti-oestrogens in the pituitary and hypothalamus of goldfish,Carassius auratus. Gen. Comp. Endocrinol. 32: 213–221.

    PubMed  Google Scholar 

  • Billard, R., Richard, M. and Breton, B. 1977. Stimulation of gonadotropin secretion after castration in rainbow trout. Gen. Comp. Endocrinol. 33: 163–165.

    PubMed  Google Scholar 

  • Bommelaer, M.C., Billard, R. and Breton, B. 1981. Changes in plasma gonadotropin after ovariectomy and estradiol supplementation at different stages at the end of the reproductive cycle in the rainbow trout,Salmo gairdneri. R. Nutr. Dev. 21: 989–997.

    Google Scholar 

  • Breton, B., Jalabert, B. and Fostier, A. 1975. Induction de décharges gonadotropes hypophysaires chez la carpe (Cyprinus carpio L.) à l'aide du citrate de cisclomyphène. Gen. Comp. Endocrinol. 25: 400–404.

    PubMed  Google Scholar 

  • Breuer, H., Schneider, H.T., Doberauer, C., Grüter, S. and Ladosky, W. 1981. Effect of oestradiol-17β and 2-hydroxyoestradiol-17β on LH concentration in plasma and COMT activity in hypothalamic nuclei of rats. Exp. Brain Res., Suppl. 3: 118–129.

    PubMed  Google Scholar 

  • Breuer, H. and Koster, G. 1974. Interaction between oestrogens and neurotransmitters at the hypophysial-hypothalamic level. J. Steroid Biochem. 5: 961–967.

    Google Scholar 

  • Chang, J.P., MacKenzie, D.S., Gould, D.R. and Peter, R.E. 1984. Effect of dopamine and norepinephrine onin vitro spontaneous and gonadotropin-releasing hormone-induced gonadotropin release by dipersed cells or fragments of the goldfish pituitary. Life Sci. 35: 2027–2033.

    PubMed  Google Scholar 

  • De Leeuw, R., Goos, H.Th., Peute, J., van Pelt, A.M.M., Burzawa-Gérard, E. and Van Oordt, P.G.W.J. 1984. Isolation of gonadotrops from the pituitary of the African catfish,Clarias lazera, morphological and physiological characterization of the purified cells. Cell. Tiss. Res. 236: 669–675.

    Google Scholar 

  • De Leeuw, R., Goos, H.J.Th. and Van Oordt, P.G.W.J. 1986a. The dopaminergic inhibition of the gonadotropin-releasing hormone-induced gonadotropin release: Anin vitro study with fragments and cell suspensions from pituitaries of the African catfish,Clarias gariepinus (Burchell). Gen. Comp. Endocrinol. 63: 171–177.

    PubMed  Google Scholar 

  • De Leeuw, R., Resink, J.W., Rooyakkers, E.J.M. and Goos, H.J.Th. 1985a. Pimozide modulates the luteinizing hormone-releasing hormone effect on gonadotropin release in the African catfish,Clarias lazera. Gen. Comp. Endocrinol. 58: 120–127.

    PubMed  Google Scholar 

  • De Leeuw, R., Smit-van Dijk, W., Zigterman, J.W.J., Van der Loo, J.C.M., Lambert, J.G.D. and Goos, H.J.Th. 1985b. Aromatase, estrogen 2-hydroxylase, and catechol-0-methyltransferase activity in isolated, cultured gonadotropic cells of mature African catfishClarias gariepinus (Burchell). Gen. Comp. Endocrinol. 60: 171–177.

    PubMed  Google Scholar 

  • De Leeuw, R., Van Asselt, L.A.C., Rupert, Th., Smit-van Dijk, W., Goos, H.J.Th. and Van Oordt, P.G.W.J. 1987b. The effects of catecholamines and catecholestrogens on gonadotropin release in the African catfish,Clarias gariepinus. Gen. Comp. Endocrinol. 66: 3.

    Google Scholar 

  • De Leeuw, R., Wurth, Y.A., Zandbergen, M.A., Peute, J. and Goos, H.J.Th. 1986b. Effects of aromatizable androgens, non-aromatizable androgens, and estrogens on gonadotropin release in castrated African catfish,Clarias gariepinus (Burchell): A physiological and ultrastructural study. Cell Tiss. Res. 243: 587–594.

    Google Scholar 

  • Dufour, S., Delerue-Le Belle, N. and Fontaine, Y.A. 1984. Evidence for pimozide and LHRH-induced gonadotropin release in estradiol-treated juvenile teleosts. Gen. Comp. Endocrinol. 53: 458.

    Google Scholar 

  • Goos, H.J.Th. 1987. Steroid feedback on pituitary gonadotropin secretion.In Reproductive Physiology of Fish. pp. 16–20. Edited by D.R. Idler, L.W. Crim and J.M. Walsh. Memorial University Press, St. John's.

    Google Scholar 

  • Herlant, M. 1960. Etude critique de deux techniques nouvelles destineés à mettre en évidence les différentes catégories présentés dans la gland pituitaire. Bull. Micro. Appl. 10: 37–44.

    Google Scholar 

  • Knuppen, R. 1981. Mediators of feed-back message: oestrogens and/or catecholestrogens? Exp. Brain Res., Suppl. 3: 108–117.

    PubMed  Google Scholar 

  • Lambert, J.G.D., Goos, H.J.Th. and Van Oordt, P.G.W.J. 1984. Aromatase activity in the brain of the rainbow trout,Salmo gairdneri. Gen. Comp. Endocrinol. 53: 459.

    Google Scholar 

  • Lloyd, T., Weisz, J. and Breakefield, X.O. 1978. The catechol estrogen, 2-hydroxyestradiol, inhibits catechol-O-methyltransferase activity in neuroblastoma cells. J. Neurochem. 31: 245–250.

    PubMed  Google Scholar 

  • Singh, T.P. and Singh, A.K. 1979. Ovarian activity, and total gonadotrophin and gonadotrophin-releasing hormone levels in response to some synthetic non-steroidal ovulating agents in the catfish,Heteropneustes fossilis (Blah). J. Endocrinol. 82: 341–345.

    PubMed  Google Scholar 

  • Timmers, R.J.M., Granneman, J.C.M., Lambert, J.G.D. and Van Oordt, P.G.W.J. 1988a. Estrogen-2-hydroxylase in the brain of the male African catfish,Clarias gariepinus. Gen. Comp. Endocrinol. (In press).

  • Timmers, R.J.M. and Lambert, J.G.D. 1987a. Aromatase and COMT activity in the brain of the African catfish,Clarias gariepinus. Gen. Comp. Endocrinol. 66: 3.

    Google Scholar 

  • Timmers, R.J.M. and Lambert, J.G.D. 1988b. Kinetic studies of catechol-0-methyltransferase from the brain of the African catfish,Clarias gariepinus Comp. Biochem. Physiol. (In press).

  • Timmers, R.J.M., Lambert, J.G.D., Peute, J., Vullings, H.G.B. and Van Oordt, P.G.W.J. 1987b. Localization of aromatase in the brain of the male African catfish,Clarias gariepinus (Burchell), by microdissection and biochemical identification. J. Comp. Neurol. 258: 368–377.

    PubMed  Google Scholar 

  • Van Putten, L.J.A., Peute, J., Van Oordt, P.G.W.J., Goos, H.J.Th. and Breton, B. 1981. Glycoprotein gonadotropin in the plasma and its cellular origin in the adenohypophysis of sham-operated and ovariectomized rainbow trout,Salmo gairdneri. Cell Tiss. Res. 218: 439–448.

    Google Scholar 

  • Zürcher, G. and Da Prada, M. 1982. Rapid and sensitive single step radiochemical assay for catechol-0-methyltransferase. J. Neurochem. 38: 191–195.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Experimental Zoology, Research Group for Endocrinology, University of Utrecht, Padualaan 8, 3584 CH, Utrecht, The Netherlands

    L. A. C. Van Asselt, R. J. M. Timmers, H. J. Th. Goos, J. G. D. Lambert & P. G. W. J. Van Oordt

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  1. L. A. C. Van Asselt
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  2. R. J. M. Timmers
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  3. H. J. Th. Goos
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  4. J. G. D. Lambert
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  5. P. G. W. J. Van Oordt
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Van Asselt, L.A.C., Timmers, R.J.M., Goos, H.J.T. et al. Catecholestrogens inhibit dopamine methylation in the gonadotrops of the African catfish,Clarias gariepinus . Fish Physiol Biochem 6, 207–212 (1989). https://doi.org/10.1007/BF01875023

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