Bioreactor magnetic fields do not affect growth and antibody productivity of GAP-A3 hybridomas
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GAP-A3 hybridoma cells grown in T-flasks exposed to the rotating magnetic field of a spinner flask stirrer base show no effect of stirrer speed in the range 0–150 rpm on their growth rate or antibody productivity. Similarly mechanically and magnetically driven spinner flasks at 48 and 70 rpm show no difference in these rates that may be attributed to the magnetic field.
KeywordsGrowth Rate Magnetic Field Antibody Productivity Stirrer Speed Spinner Flask
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- Blackman, C. F., Benane, S. G., Elliot, D. J., House, D. E., and Pollock, M. M. (1988) Influence of electromagnetic fields on the efflux of calcium ion from brain tissue in vitro. Bioelectromagnetics. 9, 215–227.Google Scholar
- Blackman, C. F., Kinney, L. S., House, D. E., and Joines, W. T. (1989). Multiple power density windows and their possible origin. Bioelectromagnetics. 10, 115–128.Google Scholar
- Cherry, R. S., and Papoutsakis, E. T. (1986). Hydrodynamic effects on cells in agitated tissue culture reactors. Bioproc. Eng. 1, 29–41.Google Scholar
- Liboff, A. R., Williams, T., Jr., Strong, D. M., and Wistar, R., Jr. (1984). Time varying magnetic fields: effect on DNA synthesis. Science. 223, 818–820.Google Scholar
- Liboff, A. R., and McLeod, B. R. (1988). Kinetics of channelized membrane ions in magnetic fields. Bioelectromagnetics. 9, 39–51.Google Scholar
- Parkinson, W. C., and Hanks, C. T., (1989). Search for cyclotron resonance in cells in vitro. Bioelectromagnetics. 10, 129–145.Google Scholar
- Reese, J. A., Jostes, R. F., and Frazier, M. E. (1988). Exposure of mammalian cells to 60-Hz magnetic or electric fields. Bioelectromagnetics. 9, 237–247.Google Scholar
- Takahashi, K., Kaneko, I., Date, M., and Fukada, E. (1986). Effect of pulsing electromagnetic fields on DNA synthesis in mammalian cells in culture. Experientia. 42, 185–186.Google Scholar
- Yen-Patton, G. P. A., Patton, W. F., Beer, D. M., and Jacobson, B. S. (1988). Endothelial cell response to pulsed electromagnetic fields: stimulation of growth rate and angiogenesis in vitro. J. Cell Physiol. 134, 37–46.Google Scholar