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Fish Physiology and Biochemistry

, Volume 15, Issue 1, pp 83–94 | Cite as

The effects of experimental anaemia on CO2 excretionin vitro in rainbow trout,Oncorhynchus mykiss

  • Kathleen M. Gilmour
  • Steve F. Perry
Article

Abstract

The effects of severe experimental anaemia on red blood cell HCO3 dehydrationin vitro were examined in rainbow trout,Oncorhynchus mykiss. After 5 days of anaemia (haematocrit=4.9±1.1%) induced by intraperitoneal injection of phenylhydrazine hydrochloride, fish displayed elevated arterial CO2 tensions (anaemic PaCO2=3.19±0.42 torrvs. control PaCO2=1.35±0.17 torr) and a significant acidosis (anaemic pHa=7.73±0.04vs. control pHa=7.99±0.04). However, after 15–20 days of anaemia (hct=6.6±0.8%) induced by blood withdrawal, the arterial CO2 tension was significantly lower than the control value, suggesting that physiological adjustments occurred within this time period to compensate for the lowered haematocrit. Compensation probably did not involve alterations in ventilation, which was unaffected by 5 days of anaemia (anaemic\(\dot V\);w=786±187 ml min−1 kg−1vs. control\(\dot V\);w=945±175 min−1 kg−1), based on indirect Fick principle measurements.

Potential adaptations to longer term anaemia at the level of the red blood cells were investigated using a radioisotopic HCO3 dehydration assay. Owing to the difference in haematocrits, the HCO3 dehydration rate for blood from anaemic fish was significantly lower than that for control fish following equilibration at the same CO2 tension. This difference was eliminated when HCO3 dehydration rates were measured on blood samples adjusted to the same haematocrit, a result which implies that the intrinsic rate of CO2 excretion at the level of the red blood cell was not ‘up-regulated’ during anaemia. The difference was also eliminated by equilibrating the blood samples with CO2 tensions appropriate for the group from which the sample was obtained,i.e., PCO2=1.4 torr for control samples and PCO2=3.2 torr for anaemic samples; each at the appropriate haematocrit. It is concluded that the elevated PaCO2 helps to reset CO2 excretion to the control level, but that some additional physiological adjustment occurs to lower the PaCO2 after 15–20 days of anaemia.

Keywords

Oncorhynchus mykiss anaemia phenylhydrazine red blood cell CO2 excretion HCO3 dehydration Cl/HCO3 exchange ventilation 

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References

  1. Blaxhall, P.C. and Daisley, K.W. 1973. Routine haematological methods for use with fish blood. J. Fish Biol. 5: 777–781.Google Scholar
  2. Boutilier, R.G., Heming, T.A. and Iwama, G.K. 1984. Physicochemical parameters for use in fish respiratory physiology.In Fish Physiology. Vol. 10A, pp. 403–430. Edited by W.S. Hoar and D.J. Randall. Academic Press, New York.Google Scholar
  3. Cameron, J.N. and Davis, J.C. 1970. Gas exchange in rainbow trout (Salmo gairdneri) with varying blood oxygen capacity. J. Fish Res. Bd. Can. 27: 1069–1085.Google Scholar
  4. Dimberg, K. 1994. The carbonic anhydrase inhibitor in trout plasma: purification and its effect on carbonic anhydrase activity and the Root effect. Fish Physiol. Biochem. 12: 381–386.Google Scholar
  5. Dejours, P. 1973. Problems of control of breathign in fishes.In Comparative Physiology: Locomotion, Respiration, Transport and Blood. pp. 117–133. Edited by L. Bolis, K. Schmidt-Nielson and S.H.P. Maddrell. American Elsevier, New York.Google Scholar
  6. Holeton, G.F. and Randall, D.J. 1967. Changes in blood pressure in the rainbow trout during hypoxia. J. Exp. Biol. 46: 297–305.Google Scholar
  7. Houston, A.H., Murad, A. and Gray, J.D. 1988. Induction of anemia in goldfish,Carassius auratus L., by immersion in phenylhydrazine hydrochloride. Can. J. Zool. 66: 729–736.Google Scholar
  8. Iwama, G.K., Boutilier, R.G., Heming, R.A., Randall, D.J. and Mazeaud, M. 1987. The effects of altering gill water flow on gas transfer in rainbow trout. Can. J. Zool. 65: 2466–2470.Google Scholar
  9. Janssen, R.G. and Randall, D.J. 1975. The effects of changes in pH and PCO2 in blood and water on breathing in rainbow trout,Salmo gairdneri. Resp. Physiol. 25: 235–245.Google Scholar
  10. Jones, D.R. 1971. The effect of hypoxia and anaemia on the swimming performance of rainbow trout (Salmo gairdneri). J. Exp. Biol. 55: 541–551.Google Scholar
  11. Lane, H.C., Rolfe, A.E. and Nelson, J.R. 1981. Changes in the nucleotide triphosphate/haemoglobin and nucleotide triphosphate/red cell ratios of rainbow trout,Salmo gairdneri Richardson, subjected to prolonged starvation and bleeding. J. Fish Biol. 18: 661–668.Google Scholar
  12. Maren, T.H. 1967. Carbonic anhydrase: chemistry, physiology and inhibition. Physiol. Rev. 47: 598–791.Google Scholar
  13. Nikinmaa, M. and Vihersaari, L. 1993. Pre- and postbranchial carbon dioxide content of rainbow trout (Oncorhynchus mykiss) blood after catecholamine injection. J. Exp. Biol. 180: 315–321.Google Scholar
  14. Perry, S.F. 1986. Carbon dioxide excretion in fishes. Can. J. Zool. 64: 565–572.Google Scholar
  15. Perry, S.F., Booth, C. and McDonald, D.G. 1985, Physiological studies using the perfused head of rainbow trout I. A critical evaluation of gas transfer, acid-base regulation and hemodynamics. Am. J. Physiol. 249: R255-R261.Google Scholar
  16. Perry, S.F., Davie, P.S., Daxboeck, C. and Randall, D.J. 1982. A comparison of CO2 excretion in a spontaneously ventilating blood-perfused trout preparation and saline-perfused gill preparations: contribution of the branchial epithelium and red blood cell. J. Exp. Biol. 101: 47–60.Google Scholar
  17. Perry, S.F. and Gilmour, K.M. 1993. An evaluation of factors limiting carbon dioxide excretion by trout red blood cellsin vitro. J. Exp. Biol. 180: 39–54.Google Scholar
  18. Perry, S.F. and Laurent, P. 1990. The role of carbonic anhydrase in carbon dioxide excretion, acid-base balance and ionic regulation in aquatic gill breathers.In Transport, Respiration and Excretion: Comparative and Environmental Aspects. pp. 39–57. Edited by J.P. Truchot and B. Lahlou. Karger, Basel.Google Scholar
  19. Perry, S.F. and McDonald, D.G. 1993. Gas exchange.In The Physiology of Fishes, pp. 251–278. Edited by D.H. Evans. CRC Press, Boca Raton.Google Scholar
  20. Perry, S.F., Wood, C.M., Thomas, S. and Walsh, P.J. 1991. Adrenergic inhibition of carbon dioxide excretion by trout red blood cellsin vitro is mediated by activation of Na+/H+ exchange. J. Exp. Biol. 157: 367–380.Google Scholar
  21. Perry, S.F., Wood, C.M., Walsh, P.J. and Thomas, S. 1995. Fish red blood cell carbon dioxide excretionin vitro: a comparative study. Comp. Biochem. Physiol. A (In press).Google Scholar
  22. Playle, R.C., Munger, R.S. and Wood, C.M. 1990. Effects of catecholamines on gas exchange and ventilation in rainbow trout (Salmo gairdneri). J. Exp. Biol. 152: 353–367.Google Scholar
  23. Smith, C.E., McLain, L.R. and Zaugg, W.S. 1971. Phenylhydrazine-induced anemia in chinook salmon. Toxicol. Appl. Pharmacol. 20: 73–81.Google Scholar
  24. Soivio, A., Nyholm, K. and Westman, K. 1975. A technique for repeated blood sampling of the blood of individual resting fish. J. Exp. Biol. 62: 207–217.Google Scholar
  25. Steffensen, J.F., Tufts, B.L. and Randall, D.J. 1987. Effect of burst swimming and adrenaline infusion on O2 consumption and CO2 excretion in rainbow trout,Salmo gairdneri J. Exp. Biol. 131: 427–434.Google Scholar
  26. Tucker, V.A. 1967. Method for oxygen content and dissociation curves on microliter blood samples. J. Appl. Physiol. 23: 410–414.Google Scholar
  27. Vorger, P. and Ristori, M.-T. 1985. Effects of experimental anemia on the ATP content and the oxygen affinity of the blood in the rainbow trout (Salmo gairdnerii). Comp. Biochem. Physiol. 82A: 221–224.Google Scholar
  28. Wolf, K. 1963. Physiological salines for freshwater teleosts. Progr. Fish. Cult. 25: 135–140.Google Scholar
  29. Wood, C.M. 1994. HCO3 dehydration by the blood of rainbow trout following exhaustive exercise. Resp. Physiol. 98: 305–318.Google Scholar
  30. Wood, C.M., McDonald, D.G. and McMahon, B.R. 1982. The influence of experimental anaemia on blood acid-base regulationin vivo andin vitro in the starry flounder (Platichthys stellatus) and the rainbow trout (Salmo gairdneri). J. Exp. Biol. 96: 221–237.Google Scholar
  31. Wood, C.M., McMahon, B.R. and McDonald, D.G. 1979. Respiratory, ventilatory, and cardiovascular responses to experimental anaemia in the starry flounder,Platichthys stellatus. J. Exp. Biol. 82: 139–162.Google Scholar
  32. Wood, C.M. and Munger, R.S. 1994. Carbonic anhydrase injection provides evidence for the role of blood acid-base status in stimulating ventilation after exhaustive exercise in rainbow trout. J. Exp. Biol. 194: 225–253.Google Scholar
  33. Wood, C.M. and Perry, S.F. 1985. Respiratory, circulatory, and metabolic adjustments to exercise in fish.In Circulation, Respiration, Metabolism. pp. 2–22. Edited by R. Gilles, Springer-Verlag, Berlin.Google Scholar
  34. Wood, C.M. and Perry, S.F. 1991. A newin vitro assay for carbon dioxide excretion by trout red blood cells: effects of catecholamines. J. Exp. Biol. 157: 349–366.Google Scholar
  35. Wood, C.M., Perry, S.F., Walsh, P.J. and Thomas, S. 1994. HCO3 dehydration by the blood of an elasmobranch in the absence of a Haldane effect. Resp. Physiol. 98: 319–337.Google Scholar
  36. Wood, C.M. and Randall, D.J. 1971. The effect of anemia on ion exchange in the southern flounderParalichthys lethostigma. Comp. Biochem. Physiol. 39: 391–402.Google Scholar
  37. Wood, C.M. and Simmons, H. 1994. The conversion of plasma HCO3 to CO2 by rainbow trout red blood cellsin vitro: adrenergic inhibition and the influence of oxygenation status. Fish Physiol. Biochem. 12: 445–454.Google Scholar

Copyright information

© Kugler Publication bv 1996

Authors and Affiliations

  1. 1.Department of BiologyUniversity of OttawaOttawaCanada

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