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Fish Physiology and Biochemistry

, Volume 15, Issue 1, pp 41–48 | Cite as

Tyrosine hydroxylase activity and dopamine turnover of rainbow trout (Oncorhynchus mykiss) brain: the special status of the hypothalamus

  • Boris Linard
  • Sanae Bennani
  • Patrick Jego
  • Christian Saligaut
Article

Abstract

The dynamics of catecholamine (CA)-synthesis enzymes have been poorly studied in fish. Tyrosine hydroxylase (TH), the rate-limiting enzyme of CA synthesis has been only studied inin vitro conditions. In the present report thein vivo CA synthesis and the CA metabolism were studied in different regions of the forebrain of the rainbow trout. Levels of norepinephrine (NE), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC) and the rate of accumulation of 3,4-dihydroxyphenylalanine (DOPA) were determined by HPLC following a treatment with hydroxybenzylhydrazine (NSD), a potential inhibitor of DOPA decarboxylase. Kinetics of the accumulation of DOPA and of the decline of DOPAC were in agreement with those found in rat, evidencing that the accumulation of DOPA following NSD can be used in trout to quantify thein vivo enzymatic activity of tyrosine hydroxylase. Experiments using treatment with NSD or with methyl-p-tyrosine reached a same conclusion: the DA neuronal activity in trout is much higher than NE neuronal activity. However, the hypothalamus had high DA levelsvs. lowin vitro andin vivo TH activities and exhibited a low CA turnover.

Keywords

trout dopamine norepinephrine tyrosine hydroxylase olfactory bulbs hypothalamus preoptic area telencephalon 

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Copyright information

© Kugler Publication bv 1996

Authors and Affiliations

  • Boris Linard
    • 1
  • Sanae Bennani
    • 1
  • Patrick Jego
    • 1
  • Christian Saligaut
    • 1
  1. 1.Laboratoire de Physiologie des Régulations, U.R.A. CNRS 256, Equipe associée d'Endocrinologie Moléculaire des PoissonsINRA/Université de Rennes IRennes cedexFrance

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