Journal of Biomolecular NMR

, Volume 2, Issue 4, pp 395–400 | Cite as

Rapid acquisition of three-dimensional triple-resonance experiments using pulsed field gradient techniques

  • Adrian L. Davis
  • Rolf Boelens
  • Robert Kaptein
Short Communications

Summary

A rapid method for recording three-dimensional triple-resonance experiments utilising pulsed field gradient techniques is proposed, and applied to the HNCO experiment. In order to optimise the sensitivity of the method, a short phase cycle is used in conjunction with the pulsed field gradients to select the desired coherence transfer pathway. The method is demonstrated for the HU protein.

Keywords

Triple-resonance Pulsed field gradients 3D NMR Proteins 

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Copyright information

© ESCOM Science Publishers B.V 1992

Authors and Affiliations

  • Adrian L. Davis
    • 1
  • Rolf Boelens
    • 1
  • Robert Kaptein
    • 1
  1. 1.Department of Chemistry, Bijvoet Centre for Biomolecular ResearchUniversity of UtrechtUtrechtThe Netherlands

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