Journal of Biomolecular NMR

, Volume 2, Issue 4, pp 395–400 | Cite as

Rapid acquisition of three-dimensional triple-resonance experiments using pulsed field gradient techniques

  • Adrian L. Davis
  • Rolf Boelens
  • Robert Kaptein
Short Communications


A rapid method for recording three-dimensional triple-resonance experiments utilising pulsed field gradient techniques is proposed, and applied to the HNCO experiment. In order to optimise the sensitivity of the method, a short phase cycle is used in conjunction with the pulsed field gradients to select the desired coherence transfer pathway. The method is demonstrated for the HU protein.


Triple-resonance Pulsed field gradients 3D NMR Proteins 


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  1. Barker, P. and Freeman, R. (1985)J. Magn. Reson. 64, 334–338.Google Scholar
  2. Bax, A. and Ikura, M. (1991)J. Biomol. NMR,1, 99–104.PubMedGoogle Scholar
  3. Bax, A., de Jong, P.G., Mehlkopf, A.F. and Smidt, J. (1980)Chem. Phys. Lett. 69, 567–570.Google Scholar
  4. Bodenhausen, G., Kogler, H. and Ernst, R.R. (1984)J. Magn. Reson. 58, 370–388.Google Scholar
  5. Brereton, I.M., Crozier, S., Field, J. and Doddrell, D.M. (1991)J. Magn. Reson. 93, 54–62.Google Scholar
  6. Clubb, R.B., Thanabal, V. and Wagner, G. (1992)J. Magn. Reson.,97, 213–217.Google Scholar
  7. Davis, A.L., Laue, E.D., Keeler, J., Moskau, D. and Lohman, J. (1991)J. Magn. Reson. 94, 637–644.Google Scholar
  8. Davis, A.L., Keeler, J., Laue, E.D. and Moskau, D. (1992)J. Magn. Reson. 98 207–216.Google Scholar
  9. Hurd, R.E. (1990)J. Magn. Reson. 87, 422–428.Google Scholar
  10. Hurd, R.E. and John, B.K. (1991)J. Magn. Reson. 92, 658–668.Google Scholar
  11. Ikura, M., Kay, L.E. and Bax, A. (1990)Biochemistry,29, 4659–4667.PubMedGoogle Scholar
  12. Kay, L.E., Ikura, M. and Bax, A. (1990)J. Magn. Reson. 89, 496–514.Google Scholar
  13. Keeler, J. and Neuhaus, D. (1985)J. Magn. Reson. 63, 454–472.Google Scholar
  14. Marion, D. and Wüthrich, K. (1983)Biochem. Biophys. Res. Commun. 96, 967–974.Google Scholar
  15. Maudsley, A.A., Wokaun, A. and Ernst, R.R. (1978)Chem. Phys. Lett. 55, 9–14.Google Scholar
  16. Morris, G.A. and Freeman, R. (1979)J. Am. Chem. Soc. 101, 760–762.Google Scholar
  17. Tanaka, I., Appelt, K., Dijk, J., White, S.W. and Wilson, K.S. (1984)Nature,310, 376–381.PubMedGoogle Scholar
  18. Vuister, G.W., Boelens, R., Kaptein, R., Hurd, R.E., John, B. and van Zijl, P.C.M. (1991)J. Am. Chem. Soc.,113, 9688–9690.Google Scholar
  19. Vuister, G.W., Boelens, R., Kaptein, R., Burgering, M. and van Zijl, P.C.M. (1992)J. Biomol. NMR. 2, 301–305.Google Scholar
  20. Westler, W.M., Stockman, B.J. and Markley, J.L. (1988)J. Am. Chem. Soc.,110, 6256–6258.Google Scholar

Copyright information

© ESCOM Science Publishers B.V 1992

Authors and Affiliations

  • Adrian L. Davis
    • 1
  • Rolf Boelens
    • 1
  • Robert Kaptein
    • 1
  1. 1.Department of Chemistry, Bijvoet Centre for Biomolecular ResearchUniversity of UtrechtUtrechtThe Netherlands

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