Abstract
Inoculation of potato callus cultures with basidiospores of the cocoa pathogenCrinipellis perniciosa resulted in the development of a mycelium which was morphologically identical to that found in green cocoa brooms. These dual cultures could be maintained for periods of several months. The nuclear condition of this mycelium was found to be variable, in contrast to previous reports. Basidiospores of the L-biotype ofC. perniciosa were also able to form dual cultures, although a biotrophic phase in its life cycle has yet to be demonstratedin vivo. Attempts to form stable heterokaryons between genetically distinct biotrophic mycelia were unsuccessful.
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Griffith, G.W., Hedger, J.N. Dual culture ofCrinipellis perniciosa and potato callus. Eur J Plant Pathol 100, 371–379 (1994). https://doi.org/10.1007/BF01874805
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DOI: https://doi.org/10.1007/BF01874805