Abstract
Filamentous flexous partic les of unusual morphology, previously associated with several ringspot isolates, were detected also in psorosis A and psorosis B isolates by serologically specific electron microscopy using an antiserum to citrus ringspot. Upon partial purification of six ringspot, six psorosis A, and three psorosis B isolates, a specific protein of 47 kDa was detected in most cases, but two isolates (one psorosis A and one ringspot) had a 46 and a 48 kDa-protein, respectively. These differences in molecular masses were observed when purification was done from different host species or from plants co-inoculated with two isolates differing by their protein size. The three types of protein were serologically related in Western blots. Our results indicate that a common virus with different strains may be involved in psorosis A, psorosis B, and ringspot diseases.
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References
Brakke MK, Skopp RN and Lane LC (1990) Degradation of wheat streak mosaic virus capsid protein during leaf senescence. Phytopathology 80: 1401–1405
Da Graça JV, Bar-Joseph M and Derrick KS (1993) Immunoblot detection of citrus psorosis in Israel using citrus ringspot antiserum. In: Moreno P, Da Graça JV and Timmer LW (eds) Proc 12th Conf Int Organ of Citrus Virol (pp 432–434). IOCV, Riverside
Da Graça JV, Lee RF, Moreno P, Civerolo EL and Detrick KS (1991) Comparison of isolates of citrus ringspot, psorosis, and other viruslike agents of citrus. Plant Disease 75: 613–616
Derrick KS, Brlansky RH, da Graça JV, Lee RF, Timmer LW and Nguyen TK (1988) Partial characterization of a virus associated with citrus ringspot. Phytopathology 78: 1298–1301
Derrick KS, Lee RF, Hewitt BG and Barthe GA (1993) Spiroviruses: A new group of serologically diverse plant viruses associated with citrus psorosis and ringspot. In: Moreno P, da Graça JV and Timmer LW (eds) Proc 12th Conf Int Organ of Citrus Virol (pp 428–429). IOCV, Riverside
Duran-Vila N, Cambra M, Pina JA, Ballester JF and Navarro L (1988) Virus content and growth patterns of callus culturedin vitro from healthy and virus-infected citrus species. In: Timmer LW, Garnsey SM and Navarro L (eds) Proc 10th Conf Int Organ Citrus Virol (pp 310–321). IOCV, Riverside
García ML, Grau O and Sarachu AN (1991) Citrus psorosis is probably caused by a bipartite ssRNA virus. Res Virol 142: 303–311
Garnsey SM (1975) Two mechanically transmissible viruses in navel orange selections introduced from Algeria. Plant Dis Reptr 59: 689–693
Garnsey SM and Timmer LW (1980) Mechanical transmissibility of citrus ringspot virus isolates from Florida, Texas and California. In: Calavan EC, Garnsey SM and Timmer LW (eds) Proc 8th Conf Int Organ of Citrus Virol (pp 174–179), IOCV, Riverside
Garnsey SM and Timmer LW (1988) Local lesion isolate of citrus ringspot virus induces psorosis bark scaling. In: Timmer LW, Garnsey SM and Navarro L (eds) Proc 10th Conf Int Organ of Citrus Virol (pp 334–339). IOCV, Riverside
Guerri J, Moreno P and Lee RF (1990) Characterization of strains of citrus tristeza virus by peptide maps of virion coat protein. Phytopathology 80: 692–698
Levy L and Gumpf DJ (1991) Studies on the psorosis disease of citrus and preliminary characterization of a flexuous virus associated with the disease. In: Brlansky RH, Lee RF and Timmer LW (eds) Proc 11 th Conf Int Organ of Citrus Virol (pp 319–336). IOCV, Riverside
Navarro L, Juárez J, Ballester JF and Pina JA (1980) Elimination of some citrus pathogens producing psorosis-like leaf symptoms, by shoot-tip graftingin vitro. In: Calavan EC, Garnsey SM and Timmer LW (eds) Proc 8th Conf Int Organ of Citrus Virol (pp 162–166). IOCV, Riverside
Navas-Castillo J (1991) Caracterización de las enfermedades del grupo psoriasis de los cítricos y ensayos de purificación de virus asociados. Tesis Doctoral. Universitat de València, Facultad de Ciencias Biológicas, Valencia
Navas-Castillo J and Moreno P (1993) Biological diversity of citrus ringspot isolates in Spain. Plant Pathology 42: 347–357
Navas-Castillo J, Moreno P, Ballester-Olmos J, Pina JA and Hermoso de Mendoza A (1991) Detection of a necrotic strain of citrus ringspot in Star Ruby grapefruit in Spain. In: Brlansky RH, Lee RF and Timmer LW (eds) Proc 11th Conf Int Organ of Citrus Virol (pp 345–351). IOCV, Riverside
Navas-Castillo J, Moreno P, Cambra M and Derrick K (1993) Partial purification of a virus associated with a Spanish isolate of citrus ringspot. Plant Pathology 42: 339–346
Roistacher CN (1991) Graft-transmissible diseases of citrus. Handbook for detection and diagnosis. FAO, Rome
Timmer LW and Beñatena HN (1977) Comparison of psorosis and other viruses causing leaf flecking in citrus. Proc Int Soc Citriculture 3: 930–935
Wallace JM (1978) Virus and viruslike diseases. In: Reuther W, Calavan EC and Carman G (eds) The Citrus Industry. Vol. IV (pp 67–184). Univ Calif Div Agr Sci, Berkeley
Wallace JM and Drake RJ (1968) Citrange stunt and ringspot, two previously undescribed virus diseases of citrus. In: Childs JFL (ed) Proc 4th Conf Int Organ of Citrus Virol (pp 177–181). Univ Florida Press, Gainesville
Whiteside JO, Garnsey SM and Timmer LW (1988). Compendium of citrus diseases. APS, St Paul, Minnesota
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Navas-Castillo, J., Moreno, P. Filamentous flexous particles and serologically related proteins of variable size associated with citrus psorosis and ringspot diseases. Eur J Plant Pathol 101, 343–348 (1995). https://doi.org/10.1007/BF01874791
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DOI: https://doi.org/10.1007/BF01874791