European Journal of Plant Pathology

, Volume 101, Issue 3, pp 241–250 | Cite as

Detection of the German grapevine yellows (Vergilbungskrankheit) MLO in grapevine, alternative hosts and a vector by a specific PCR procedure

  • M. Maixner
  • U. Ahrens
  • E. Seemüller
Article

Abstract

A polymerase chain reaction procedure was developed which enables specific amplification of a ribosomal sequence from the mycoplasmalike organism (MLO) associated with German grapevine yellows (Vergilbungskrankheit, VK) and stolbur-related diseases of solanaceous plants. Successful amplification from all samples prepared from various cultivars collected in different viticultural areas indicates that the causal agent is a relatively homogeneous organism. Amplification was also achieved with template DNA prepared from naturally infected weeds in vineyards such asConvolvolus arvensis andSolanum nigrum, and from the planthopperHyalesthes obsoletus that was collected in the vineyards. Feeding of insects of this species on grapevine seedlings resulted in the development of typical yellows symptoms by the grapes.H. obsoletus could therefore be identified as a vector of Vergilbungskrankheit.

Key words

diagnosis mollicutes epidemiology transmission planthopper 

Abbreviations

FD

Flavescence dorée

GY

Grapevine yellows

MLO

Mycoplasmalike organism

PCR

Polymerase chain reaction

RFLP

restriction fragment length polymorphism

VK

Vergilbungskrankheit (German grapevine yellows)

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • M. Maixner
    • 1
  • U. Ahrens
    • 2
  • E. Seemüller
    • 2
  1. 1.Biologische Bundesanstalt für Land- und ForstwirtschaftInstitut für Pflanzenschutz im WeinbauBernkastel-KuesGermany
  2. 2.Biologische Bundesanstalt für Land- und ForstwirtschaftInstitut für Pflanzenschutz im ObstbauDossenheimGermany

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