Fish Physiology and Biochemistry

, Volume 5, Issue 1, pp 39–47 | Cite as

Smoltification in steelhead trout (Salmo gairdneri): Developmental aspects of plasma constituents

  • Terence M. Bradley
  • A. W. Rourke
Article

Abstract

Selected biochemical parameters were measured in the plasma of both underyearling anadromous steelhead trout (Salmo gairdneri) and underyearling residentS. gairdneri. The analyses were conducted in an effort to determine whether or not there might be changes which could be associated with the parr-smolt transformation of anadromous strains. Plasma NH+4 and plasma Na+ were assayed and plasma proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis (2D PAGE).

Ammonia was the only plasma ion to show changes with time that were different between the two strains of fish. Proteins prepared by 2D PAGE exhibited developmental changes in both migratory and nonmigratory fish. Each strain exhibited changes with time and the anadromous fish displayed patterns of proteins that were not observed in the nonanadromous strain. It is possible that certain changes in the protein constituents found in anadromous fish are associated with the processes of smoltification. The data are consistent with the notion that this developmental event occurs over an extended period of time and is not restricted to the spring.

The data suggest that there may be some changes that occur in certain plasma constituents of migratory fish beginning in the fall and continuing into the spring. The data also indicate that certain ontogenetic events that are not associated with smoltification can be ascertained by analyses of plasma.

Keywords

Salmo gairdneri smoltification plasma proteins plasma Na+ plasma NH4+ 

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References cited

  1. Bradley, T.M. and Rourke, A.W. 1984. An electrophoretic analysis of plasma proteins from juvenileOncorhynchus ishawytscha (Walbaum). J. Fish Biol. 24: 703–709.Google Scholar
  2. Bradley, T.M. and Rourke, A.W. 1985. The influences of addition of minerals to rearing water and smoltification on selected blood parameters of juvenile steelhead trout,Salmo gairdneri Richardson. Physiol. Zool. 58: 312–319.Google Scholar
  3. Dickhoff, W.W., Folmar, L.C. and Gorbman, A. 1978. Changes in plasma thyroxine during smoltification of coho salmon,Oncorhynchus kisutch. Gen. Comp. Endocrinol. 36: 229–232.PubMedGoogle Scholar
  4. Elson, P. 1957. The importance of size in the change from parr to smolt in Atlantic salmon. Can. Fish. Cult. 21: 1–6.Google Scholar
  5. Ewing, R.D. and Birks, E.K. 1982. Criteria for parr-smolt transformation in juvenile chinook salmon (Oncorhynchus tshawytscha). Aquaculture 28: 185–194.Google Scholar
  6. Fessler, J.L. and Wagner, H.H. 1969. Some morphological and biochemical changes in steelhead trout during the parr-smolt transformation. J. Fish. Res. Board Can. 26: 2823–2841.Google Scholar
  7. Folmar, L.C. and Dickhoff, W.W. 1980. The parr-smolt transformation (smoltification) and seawater adaptation in salmonids. Aquaculture 21: 1–37.Google Scholar
  8. Fontaine, M. 1960. Quelques problèmes physiologiques poseés par leSalmo salar. Intérêt de l'état de la smoltification, type de préparation au comportment migratoire. Experientia 16: 433–472.PubMedGoogle Scholar
  9. Garrels, J.I., Farrar, J.T. and Barwell IV, C.V. 1984. The QUEST System for Computer-analyzed Two-dimensional Electrophoresis of Proteins.In Two-dimensional Gel Electrophoresis of Proteins. pp. 37–91. Edited by J.E. Cellis and R. Bravo. Academic Press, New York.Google Scholar
  10. Hayashi, S. 1970. Biochemical studies on the skin of fish. II. Seasonal changes of purine content of masu salmon from parr to smolt. Bull. Jap. Soc. Sci. Fish. 37: 508–512.Google Scholar
  11. Hoar, W.S. 1939. The weight-length relationship of the Atlantic salmon. J. Fish. Res. Board Can. 4: 441–460.Google Scholar
  12. Houston, A.H. 1959. Influence of size upon the adaptation of steelhead trout (Salmo gairdneri Richardson) and coho salmon (Oncorhynchus kisutch) to seawater. J. Fish. Res. Board Can. 18: 401–415.Google Scholar
  13. Houston, A.H. and Threadgold, L.T. 1963. Body fluid regulation in smolting Atlantic salmon. J. Fish. Res. Board Can. 20: 1355–1356.Google Scholar
  14. Johnston, C.E. and Eales, J.G. 1967. Purines in the integument of the Atlantic salmon (Salmo salar) during parr-smolt transformation. J. Fish. Res. Board Can 24: 953–964.Google Scholar
  15. Johnston, C.E. and Eales, J.G. 1968. Influence of temperature and photoperiod on guanine and hypoxanthine levels in skin and scales of Atlantic salmon (Salmo salar) during parr-smolt transformation. J. Fish. Res. Board Can 25: 1901–1909.Google Scholar
  16. Johnston, C.E. and Eales, J.G. 1970. Influence of body size on silvering of Atlantic salmon (Salmo salar) during parr-smolt transformation. J. Fish. Res. Board Can. 27: 983–987.Google Scholar
  17. Kubo, T. 1955. Changes of some characteristics of blood of smolts ofOncorhynchus masou during migration. Bull. Far. Fish. Hokkaido Univ. 4: 138–148.Google Scholar
  18. Langdon, T.S. and Thorpe, J.E. 1985. The ontogeny of smoltification: developmental patterns of gill Na+/K+ ATPase, SDH and chloride cells in juvenile Atlantic salmon,Salmo salar L. Aquaculture 45: 83–96.Google Scholar
  19. McBride, J.R., Higgs, D.A., Fagerlund, U.H.M. and Bradley, J.T. 1982. Thyroid and steroid hormones: potential for control of growth and smoltification of salmonids. Aquaculture 28: 201–209.Google Scholar
  20. Miles, H.M. and Smith, L.S. 1968. Ionic regulation in migrating juvenile coho salmonOncorhynchus kisutch. Comp. Biochem. Physiol. 26: 381–398.Google Scholar
  21. Olin, T. and von der Decken, A. 1986. High mobility group proteins in skeletal muscle of pre- and post-smolt salmon (Salmo salar). Comp. Biochem. Physiol. 83: 763–765.Google Scholar
  22. Piper R.G., McElwain, I.B., Orme, L.E., McCraren, J.P., Fowler, L.G. and Leonard, J.R. 1982. Fish Hatchery Management. United States Department of the Interior. Washington, DC.Google Scholar
  23. Saunders, R.L., Henderson, E.B. and Harmon, P.R. 1985. Effects of photoperiod on juvenile growth and smolting of Atlantic salmon and subsequent survival and growth in sea cages. Aquaculture 45: 55–66.Google Scholar
  24. Sung, Z.R. 1984. Application of two-dimensional gel electrophoresis in studies of gene expression during early plant development.In Two-dimensional gel electrophoresis of proteins. pp. 397–413. Edited by J.E. Cellis and R. Bravo. Academic Press, New York.Google Scholar
  25. Vanstone, W.E. and Markert, J.R. 1968. Some morphological and biochemical changes in coho salmonOncorhynchus kisutch during the parr-smolt transformation. J. Fish. Res. Board Can. 25: 2403–2418.Google Scholar
  26. Wedemeyer, G.A., Saunders, R.L. and Clarke, W.C. 1980. Environmental factors affecting smoltification and early marine survival of anadromous salmonids. Mar. Fish. Rev. 42: 1–15.Google Scholar
  27. Zaugg, W.S. 1982. Some changes in smoltification and seawater adaptability of salmonids resulting from environmental and other factors. Aquaculture 28: 143–151.Google Scholar
  28. Zaugg, W.S. and McLain, L.R. 1972. Changes in gill adenosine-triphosphatase activity associated with parr-smolt transformation in steelhead trout, coho, and spring chinook salmon. J. Fish. Res. Board Can. 29: 161–171.Google Scholar
  29. Zaugg, W.S. and McLain, L.R. 1976. Influence of water temperature on gill sodium, potassium stimulated ATPase activity in juvenile coho salmon (Oncorhynchus kisutch). Comp. Biochem. Physiol. 54A: 419–421.Google Scholar
  30. Zaugg, W.S. and Wagner, H.H. 1973. Gill ATPase activity related to parr-smolt transformation and migration in steel-head trout (Salmo gairdneri): influence of photoperiod and temperature. Comp. Biochem. Physiol. 45B: 955–965.Google Scholar

Copyright information

© Kugler Publication 1988

Authors and Affiliations

  • Terence M. Bradley
    • 1
  • A. W. Rourke
    • 2
  1. 1.Department of Fisheries, Animal and Veterinary SciencesUniversity of Rhode IslandKingston
  2. 2.Department of Biological SciencesUniversity of IdahoMoscow

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