European Journal of Plant Pathology

, Volume 101, Issue 5, pp 535–539 | Cite as

The transmission by nematodes of tobraviruses is not determined exclusively by the virus coat protein

  • Stuart A. MacFarlane
  • Derek J. F. Brown
  • John F. Bol
Research Articles

Abstract

The coat protein gene of the nematode non-transmissible, SP5 isolate of pea early-browning tobravius was replaced with that of the highly nematode transmissible, PPK20 isolate of tobacco rattle tobravirus. Plants were infected with the recombinant virus when mechanically inoculated and the virus invaded the plants systemically. However, although the PPK20 isolate of TRV was transmitted by nematodes from these plants, the recombinant virus was not transmitted. Therefore, the virus coat protein is not the exclusive determinant of nematode transmission.

Key words

Pea early-browning virus recombination tobacco rattle virus Trichodorus Paratrichodorus 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Angenent GC, linthorst HJM, Van Belkum AF, Cornelissen BJC and Bol JF (1986) RNA2 of tobacco rattle virus strain TCM encodes an unexpected gene. Nucleic Acids Research 14: 4673–4682Google Scholar
  2. Angenent GC, Posthumus E, Brederode FT and Bol JF (1989) Geneome structure of tobacco rattle virus strain PLB: further evidence on the occurrence of RNA recombination among tobraviruses. Virology 171:271–274Google Scholar
  3. Atreya CD, Racah B and Pirone TP (1990) A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus. Virology 178:161–165Google Scholar
  4. Atreya CD, Atreya PL, Thombury DW and Pirone TP (1992) Sitedirected mutations in the potyvirus HC-PRO gene affect helper component activity, virus accumulation, and symptom expression in infected tobacco plants. Virology 191:106–111Google Scholar
  5. Bergh ST, Koziel MG, Huang S-C, Thomas RA, Gilley DP and Siegel A (1985) The nucleotide sequence of tobacco rattle virus RNA2 (CAM strain). Nucleic Acids Research 13:8507–8518Google Scholar
  6. Boccara M, Hamilton WDO and Baulcombe DC (1986) The organisation and interviral homologies of genes at the 3′ end of tobacco rattle virus RNA1. European Molecular Biology Organisation Journal 5:223–229Google Scholar
  7. Brown DJF, Pleog AT and Robinson DJ (1989) A review of reported associations betweenTrichodorus andParatrichodorus species (Nematoda: Trichodoridae) and tobraviruses with a description of laboratory methods for examining virus transmission by trichodorids. Revue de Nematologie 12:235–241Google Scholar
  8. Evans IR and Zettler FW (1970) Aphid and mechanical transmission properties of bean yellow mosaic virus isolates. Phytopathology 60:1170–1174Google Scholar
  9. Goulden MG, Lomonossoff GP, Davies JW and Wood KR (1990) The complete nucleotide sequence of PEBV RNA2 reveals the presence of a novel open reading frame and provides insights into the structure of tobraviral subgenomic promoters. Nucleic Acids Research 18:4507–4512Google Scholar
  10. Goulden MG, Lomonossoff GP, Wood KP and Davies JW (1991) A model for the generation of tobacco rattle virus (TRV) anomalous isolates: pea early-browning virus RNA-2 acquires TRV sequences from both RNA-1 and RNA-2. Journal of General Virology 72:1751–1754Google Scholar
  11. Harrison BD (1966) Further studies on a British form of pea earlybrowning virus. Annals of Applied Biology 57: 121–129Google Scholar
  12. Harrison BD and Woods RD (1966) Serotypes and particle dimensions of tobacco rattle viruses from Europe and America. Virology 28:610–620Google Scholar
  13. Harrison BD and Robinson DJ (1986) Tobraviruses. In: Van Regenmortel MHV and Fraenkel-Conrat H. (eds) The Plant Viruses Vol 2 (pp 339–369). Plenum Publishing Company, New YorkGoogle Scholar
  14. Legorburu FJ, Robinson DJ, Torrance L, Ploeg AT and Brown DJF (1992) Surface features of the patticles of tobacco rattle virus related to its transmissibility by trichodorid nematodes. Proceedings of the European Association for Potato Research, Virology Section, Congress No. 7 pp. 47–51Google Scholar
  15. MacFarlane SA, Taylor SC, King DI, Hughes G and Davies JW (1989) Pea early-browning virus RNA1 encodes four polypeptides incuding a putative zinc-finger protein. Nucleic Acids Research 17:2245–2260Google Scholar
  16. MacFarlane SA, Wallis CV, Taylor SC, Goulden MG, Wood KR and Davies JW (1991) Construction and analysis of infectious transcripts synthesized from full-length cDNA clones of both genomic RNAs of pea early-browning virus. Virology 182: 124–129Google Scholar
  17. MacFarlane SA, Gilmer D and Davies JW (1992) Efficient inoculation with CaMV 35S promoter-driven DNA clones of the tobravirus PEBV. Virology 187:829–831Google Scholar
  18. MacFarlane SA, Matthis A and Bol JF (1994) Heterologous encapsidation of recombinant PEBV. Journal of General Virology 75: 1423–1429Google Scholar
  19. Pirone TP (1991) Viral genes and gene products that determine insect transmissibility. Seminars in Virology 2:81–87Google Scholar
  20. Ploeg AT, Brown DJF and Robinson DJ (1992a) The association between species ofTrichodorus andParatrichodorus vector nematodes and serotypes of tobacco rattle tobravirus. Annals of Applied Biology 121:619–630Google Scholar
  21. Ploeg AT, Brown DJF and Robinson DJ (1992b) Acquisition and subsequent transmission of tobacco rattle virus isolates byParatrichodorus andTrichodorus nematode species. Netherlands Journal of Plant Pathology 98:291–300Google Scholar
  22. Ploeg AT, Robinson DJ and Brown DJF (1993) RNA-2 of tobacco rattle virus encodes the determinants of transmissibility by trichodorid vector nematodes. Journal of General Virology 74: 1463–1466Google Scholar
  23. Robinson DJ, Hamilton WDO, Harrison BD and Baulcombe DC (1987) Two anomalous tobravirus isolates: evidence for RNA recombination in nature. Journal of General Virology 68:2551–2561Google Scholar

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Stuart A. MacFarlane
    • 1
  • Derek J. F. Brown
    • 1
  • John F. Bol
    • 2
  1. 1.Scottish Crop Research InstituteDundeeScotland, UK
  2. 2.Gorlaeus LaboratoriesLeiden UniversityLeidenThe Netherlands

Personalised recommendations