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Comparison of two anti-T-2 toxin immunoglobulins by direct ELISA

  • Screening and Assay Methods
  • Published:
Biotechnology Techniques

Summary

Monoclonal anti-T-2 IgGs produced from 12C12 and 15H6 hybridomas were compared by enzyme-linked immunosorbent assay. Binding activity was linear from 0.005 to 0.25 μg protein/ml with 12C12 and from 0.005 to 0.09 μg protein/ml with 15H6. The quantity of T-2 toxin (μg protein/ml) required for one-half maximum binding activity of 15H6 (0.0875 μg protein/ml) was approximately 68% that of 12C12 (μg protein/ml).

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Authors and Affiliations

  1. Southern Research Institute, P.O. Box 55305, 35255-5305, Birmingham, AL

    Douglas B. Rivers, F. Reese Frazer III & Daniel R. Coleman

Authors
  1. Douglas B. Rivers
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  2. F. Reese Frazer III
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  3. Daniel R. Coleman
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Rivers, D.B., Frazer, F.R. & Coleman, D.R. Comparison of two anti-T-2 toxin immunoglobulins by direct ELISA. Biotechnol Tech 2, 69–72 (1988). https://doi.org/10.1007/BF01874211

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  • DOI: https://doi.org/10.1007/BF01874211

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