Biotechnology Techniques

, Volume 2, Issue 1, pp 63–68 | Cite as

Direct screening for acid phosphatase production on bcip-agar plates

  • Donna M. Gibson
  • Alice A. Christen
  • Edward J. Mullaney
Screening and Assay Methods


A direct screening procedure for detection of acid phosphatase activity was established using medium containing 5-bromo-4-chloro-3-indolyl phosphate. Production of acid phosphatase could be suppressed by adding phosphate. This acid phosphatase activity as well as studying its regulation.


Phosphate Phosphatase Activity Acid Phosphatase Screening Procedure Acid Phosphatase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Science & Technology Letters 1988

Authors and Affiliations

  • Donna M. Gibson
    • 1
  • Alice A. Christen
    • 1
  • Edward J. Mullaney
    • 1
  1. 1.USDA-ARS-SRRCNew Orleans

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