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Journal of Muscle Research & Cell Motility

, Volume 13, Issue 2, pp 199–205 | Cite as

Effects of ethylene glycol on the kinetics of contraction on flash photolysis of caged ATP in rat psoas muscle fibres

  • K. Horiuti
  • T. Sakoda
  • M. Takei
  • K. Yamada
Papers

Summary

ATP (1–1.2mm) was photoreleased from caged ATP (5mm) in skinned fibres from rat psoas muscle at 15–17° C, to examine the effects of ethylene glycol (EG; 20% in solvent) on the kinetics of isometric contraction. Muscle fibres were stretched by 0.5–2% before photolysis, so that force just before photolysis was almost equal to the steady-state force after photolysis. At the phase of steady-state contraction, force and 500 Hz-stifmess in the presence of EG were 50% and 70% of the controls, respectively, resulting in a higher stiffness-to-force with EG, as reported previously. Following photolysis, force fell before rising to a steady-state plateau. The estimated rate constant of the force decay was approximately 90 s−1, and in the presence of EG was 80–85% of the control. This suggested a small effect of EG on the crossbridge detachment induced by ATP. The rate of force redevelopment was approximately 70 s−1, and EG decreased this rate to 50% of the control. This suggested that EG greatly slows the transition of the crossbridges from the detached state to the reattached force-producing state. The time course of the stiffness signals was consistent with this interpretation. The high stiffness-to-force ratio with EG indicated that EG not only reduces the rate constants which were directly examined in this study but also modifies other aspects of the crossbridge reaction.

Keywords

Ethylene Cage Muscle Fibre Ethylene Glycol Isometric Contraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ando, T. &Asai, H. (1977) The effects of solvent viscosity on the kinetic parameters of myosin and heavy meromyosin ATPase.J. Bioenerg. Biomembr. 9, 283–8.Google Scholar
  2. Barman, T. E., Hillaire, D. &Travers, F. (1983) Evidence for the two-step binding of ATP to myosin subfragment 1 by the rapid-flow-quench method.Biochem. J. 209, 617–26.Google Scholar
  3. Biosca, J. A., Travers, F. &Barman, T. E., Bertrand, R., Audemard, E. &Kassab, R. (1985) Transient kinetics of adenosine 5′-triphosphate hydrolysis by covalently crosslinked actomyosin complex in water and 40% ethylene glycol by the rapid flow quench method.Biochemistry 24, 3814–20.Google Scholar
  4. Clarke, M. L., Rodger, C. D. &Tregear, R. T. (1984) Modification of crossbridge states by ethylene glycol in insect flight muscle.J. Muscle Res. Cell Motil. 5, 81–96.Google Scholar
  5. Endo, M., Kitazawa, T., Iino, M. &Kakuta, Y. (1979) Effect of ‘viscosity’ of the medium on mechanical properties of skinned skeletal muscle fibres. InCross-Bridge Mechanism in Muscle Contraction (edited by Sugi, H. & Pollack, G. H.) pp. 365–76. Tokyo: University of Tokyo Press.Google Scholar
  6. Ferenczi, M. A., Homsher, E. &Trentham, D. R. (1984) The kinetics of magnesium adenosine triphosphate cleavage in skinned muscle fibres of the rabbit.J. Physiol. 352, 579–99.Google Scholar
  7. Ford, L. E., Huxley, A. F. &Simmons, R. M. (1986) Tension transients during the rise of tetanic tension in frog muscle fibres.J. Physiol. 372, 595–609.Google Scholar
  8. Gekko, K. &Timasheff, S. N. (1981) Mechanism of protein stabilization by glycerol: preferential hydration in glycerol-water mixtures.Biochemistry 20, 4667–76.Google Scholar
  9. Goldman, Y. E. (1987) Kinetics of the actomyosin ATPase in muscle fibres.Ann. Rev. Physiol. 49, 637–54.Google Scholar
  10. Goldman, Y. E., Hibberd, M. G. &Trentham, D. R. (1984a) Relaxation of rabbit psoas muscle fibres from rigor by photochemical generation of adenosine-5′-triphosphate.J. Physiol. 354, 577–604.Google Scholar
  11. Goldman, Y. E., Hibberd, M. G. &Trentham, D. R. (1984b) Initiation of active contraction by photogeneration of adenosine-5′-triphosphate in rabbit psoas muscle fibres.J. Physiol. 354, 605–24.Google Scholar
  12. Hibberd, M. G. &Trentham, D. R. (1986) Relationships between chemical and mechanical events during muscular contraction.Ann. Rev. Biophys. Biophys. Chem. 15, 119–61.Google Scholar
  13. Hibberd, M. G., Dantzig, J. D., Trentham, D. R. &Goldman, Y. E. (1985) Phosphate release and force generation in skeletal muscle fibres.Science 228, 1317–19.Google Scholar
  14. Homsher, E. &Millar, N. C. (1990) Caged compounds and striated muscle contraction.Ann. Rev. Physiol. 52, 875–96.Google Scholar
  15. Horiuti, K., Somlyo, A. V., Goldman, Y. E. &Somlyo, A. P. (1989) Kinetics of contraction initiated by flash photolysis of caged adenosine triphosphate in tonic and phasic smooth muscles.J. Gen. Physiol. 94, 769–81.Google Scholar
  16. Kaplan, J. H., Forbush III, B. &Hoffman, J. F. (1978) Rapid photolytic release of adenosine 5′-triphosphate from a protected analogue: utilization by the Na:K pump of human red blood cell ghosts.Biochemistry 17, 1929–35.Google Scholar
  17. Kawai, M. &Brandt, P. W. (1980) Sinusoidal analysis: a high resolution method for correlating biochemical reactions with physiological processes in activated skeletal muscles of rabbit, frog and crayfish.J. Muscle Res. Cell Motil 1, 279–303.Google Scholar
  18. Kodama, T. (1985) Thermodynamic analysis of muscle ATPase mechanism.Physiol. Rev. 65, 467–551.Google Scholar
  19. Marston, S. B. &Tregear, R. T. (1984) Modification of the interactions of myosin with actin and 5′-adenylyl imidodiphosphate by substitution of ethylene glycol for water.Biochem. J. 217, 169–77.Google Scholar
  20. Maruyama, T., Kometani, K. &Yamada, K. (1989) Modification of the contractile properties of rabbit skeletal muscle by ethylene glycol.J. Biochem. 105, 1009–13.Google Scholar
  21. Nakagawa, T. &Oyanagi, Y. (1980) Program system SALS for non-linear least-squares fitting in experimental science. InRecent Developments in Statistical Inference and Data Analysis (edited by Matusita, K.) pp. 221–25. Holland: North Holland Publishing.Google Scholar
  22. Rapp, G. &Güth, K. (1988) A low cost high intensity flash device for photolysis experiments.Pflügers Arch. 411, 200–3.Google Scholar

Copyright information

© Chapman & Hall 1992

Authors and Affiliations

  • K. Horiuti
    • 1
  • T. Sakoda
    • 1
  • M. Takei
    • 1
  • K. Yamada
    • 1
  1. 1.Department of PhysiologyMedical College of OitaOitaJapan

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