Journal of Muscle Research & Cell Motility

, Volume 13, Issue 2, pp 127–131 | Cite as

Mutant actin: A dead end?

  • Emma S. Hennessey
  • Andrew Harrison
  • Douglas R. Drummond
  • John C. Sparrow
News and Views


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aspenstrom, P. &Karlsson, R (1991) Interference with myosin subfragment-1 binding by site-directed mutagenesis of actin.Eur. J. Biochem. 200, 35–41.Google Scholar
  2. Atkinson, S. J. &Stewart, M. (1991) Molecular basis of myosin assembly: coiled-coil interactions and the role of charge periodicities. InMotor Proteins (edited by Cross, R. A. & Kendrick-Jones, J.).J. Cell Sci. 14 (Suppl.), 7–10.Google Scholar
  3. Bouche, M., Goldfine, S. M. &Fischman, D. A. (1988) Posttranslational incorporation of contractile proteins into a cell-free system.J. Cell Biol. 107, 587–96.Google Scholar
  4. Cook, R. K., Sheff, D. R. &Rubenstein, P. A. (1991) Unusual metabolism of the yeast actin amino terminus.J. Biol. Chem. 266, 16825–33.Google Scholar
  5. Cooper, J. A. &Pollard, T. D. (1982) Methods to measure actin polymerisation.Methods Enzymol. 85, 182–210.Google Scholar
  6. Drummond, D. R., Hennessey, E. S. &Sparrow, J. C. (1991) Characterisation of missense mutations in theAct88F gene ofDrosophila melanogaster.Mol. Gen. Genet. 226, 70–80.Google Scholar
  7. Frankel, S., Condeelis, J. &Leinwand, L. (1990) Expression of actin inEscherichia coli.J. Biol. Chem. 265, 17980–7.Google Scholar
  8. Fyrberg, E. A., Mahaffey, J. W., Bond, B. J. &Davidson, N. (1983) Transcripts of the sixDrosophila actin genes accumulate in a stage and tissue specific manner.Cell 33, 115–23.Google Scholar
  9. Fyrberg, E., Beall, C. &Fyrberg, C. C. (1991) From genes to tensile forces: genetic dissection of contractile protein assembly and function. InMotor Proteins (edited by Cross, R. A. & Kendrick-Jones, J.).J. Cell Science 14 (Suppl.), 27–30.Google Scholar
  10. Geyer, P. K. &Fyrberg, E. A. (1986) 5′-Flanking sequence required for regulated expression of a muscle specificDrosophila melanogaster actin gene.Mol. Cell. Biol. 6, 3388–96.Google Scholar
  11. Hennessey, E. S. (1989) Biochemical studies on actin expressedin vitro from theDrosophila Actin88F gene. DPhil thesis, University of York.Google Scholar
  12. Hennessey, E. S., Drummond, D. R. &Sparrow, J. C. (1991) Post-translational processing of the amino terminus affects actin function.Eur. J. Biochem. 197, 345–52.Google Scholar
  13. Hiromi, Y. &Hotta, Y. (1985) Actin gene mutations inDrosophila; heat shock activation in the indirect flight muscles.EMBO J. 4, 1681–7.Google Scholar
  14. Hiromi, Y., Okamoto, H., Gehring, W. J. &Hotta, Y. (1986) Germline transformation withDrosophila mutant actin genes induces constitutive expression of heat shock proteins.Cell 44, 293–301.Google Scholar
  15. Hitchcock-DeGregori, S. E., Sampath, P. &Pollard, T. D. (1988) Tropomyosin inhibits the rate of actin polymerisation by stabilising actin filaments.Biochemistry 27, 9182–5.Google Scholar
  16. Kabsch, W., Mannherz, H. G., Suck, D., Pal E. F. &Holmes, K. C. (1990) Atomic structure of the actin: DNase I complex.Nature 347, 37–44.Google Scholar
  17. Korn, E. D., Carlier, M.-F. &Pantaloni, D. (1987) Actin polymerisation and ATP hydrolysis.Science 238, 638–44.Google Scholar
  18. Mahaffey, J. W., Coutu, M. D., Fyrberg, E. A. &Inwood, W. (1985) The flightlessDrosophila mutant ‘raised’ has two genetic lesions affecting accumulation of myofibrillar proteins in flight muscles.Cell 40, 101–10.Google Scholar
  19. Maynard Smith, J., Bozcuk, A. N. &Tebbutt, S. (1970) Protein turnover in the adultDrosophila.J. Insect Physiol. 16, 601–13.Google Scholar
  20. Miller, L. K. (1988) Baculoviruses as gene expression vectors.Ann. Rev. Microbiol. 42, 177–99.Google Scholar
  21. Okamoto, H., Hiromi, Y., Ishikawa, E., Yamada, T., Isoda, K., Maekawa, H. &Hotta, Y. (1986) Molecular characterization of mutant actin genes which induce heat shock proteins inDrosophila flight muscles.EMBO J. 5, 589–96.Google Scholar
  22. Rubenstein, P., Smith, P., Deuchler, J. &Redman, K. (1981) NH2-Terminal acetylation ofDictyostelium discoideum actin in a cell-free protein-synthesising system.J. Biol. Chem. 256, 8149–55.Google Scholar
  23. Rubenstein, P. A., Solomon, L. R. &Martin, D. J. (1988) Specificity of the actin amino terminal processing protease. InMolecular Biology of Intracellular Protein Sorting and Organelle Assembly (edited by Bradshaw, R. A.) pp. 99–104. New York, Alan R. Liss.Google Scholar
  24. Rubenstein, P. A., Solomon, L. R., Solomon, T. &Gay, L. (1989) Actin structure-function relationshipsin vitro using oligodeoxynucleotide directed site-specific mutagenesis.Cell Motil. Cytoskel. 14, 35–9.Google Scholar
  25. Saide, J. D., Chin-Bow, S., Hogan-Sheldon, J., Busquets-Turner, L., Vigoreaux, J. O., Valgiersdottir, K. &Pardue, M. L. (1989) Characterisation of components of Z-bands in the fibrillar flight muscle ofDrosophila melanogaster.J. Cell Biol. 109, 2157–67.Google Scholar
  26. Shortle, D., Haber, J. E. &Botstein, D. (1982) Lethal disruption of the yeast actin gene by integrative DNA transformation.Science 217, 371–3.Google Scholar
  27. Solomon, L. R. &Rubenstein, P. A. (1987) Studies on the role of actin's N7-methylhistidine using oligonucleotidedirected site-specific mutagenesis.J. Biol. Chem. 262, 11382–8.Google Scholar
  28. Solomon, T. L., Solomon, L. R., Gay, L. S., &Rubenstein, P. A. (1988) Studies on the role of actin's aspartic acid 3 and aspartic acid 11 using oligonucleotide-directed sitespecific mutagenesis.J. Biol. Chem. 263, 19662–9.Google Scholar
  29. Sparrow, J., Drummond, D., Peckham, M., Hennessey, E. &White, D. (1991) Protein engineering and the study of muscle contraction inDrosophila flight muscles. InMotor Proteins (edited by Cross, R. A. & Kendrick-Jones, J.).J. Cell Sci. 14 (Suppl.), 73–8.Google Scholar
  30. Sutoh, K., Ando, M., Sutoh, K. &Toyoshima, Y. Y. (1991) Site-directed mutations ofDictyostelium actin: Disruption of a negative charge cluster at the N-terminus.Proc. Natl Acad. Sci. USA 88, 7711–4.Google Scholar
  31. Weigt, T. C., Schoepper, B. &Wegner, A. (1990) Tropomyosintroponin complex stabilises the pointed ends of actin filaments against polymerisation and depolymerisation.FEBS Lett. 260, 266–8.Google Scholar

Copyright information

© Chapman & Hall 1992

Authors and Affiliations

  • Emma S. Hennessey
    • 1
  • Andrew Harrison
    • 1
  • Douglas R. Drummond
    • 1
  • John C. Sparrow
    • 1
  1. 1.Department of BiologyUniversity of YorkHeslingtonUK

Personalised recommendations