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Effects of membrane potential on Na cotransports in eel intestinal brush-border membrane vesicles: Studies with a fluorescent dye

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Summary

The Na-dependent transport of a number of organic molecules (d-glucose,l-proline,l-alanine,l-phenylalanine) in brush-border membrane vesicles isolated from the intestine of the eel (Anguilla anguilla) was monitored by recording the fluorescence quenching of the voltage-sensitive cyanine dye 3,3′-diethylthiacarbocyanine iodide (DiS-C2(5)). The experimental approach consisted of: a) generating an inside-negative membrane potential mimicking “in vivo” conditions: b) measuring the rate of membrane potential decay (i.e., the rate of fluorescence quenching decay) due to Na-neutral substrate cotransport. Rates of membrane potential decay showed saturation on substrate concentration andK app values (the substrate concentration giving 50% of the maximal rate) were estimated for Na-dependent transport ofd-glucose (0,099mm),l-alanine (0.516mm),l-proline (0.118mm) andl-phenylalanine (2.04mm). The influence of an inside-negative membrane potential on the affinity of the transporter for glucose and for sodium is discussed.

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Authors and Affiliations

  1. Laboratorio di Fisiologia, Dipartimento di Biologia, Università de Lecce, 73100, Lecce, Italy

    Giuseppe Cassano, Michele Maffia, Sebastiano Vilella & Carlo Storelli

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  1. Giuseppe Cassano
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  2. Michele Maffia
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  3. Sebastiano Vilella
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  4. Carlo Storelli
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Cassano, G., Maffia, M., Vilella, S. et al. Effects of membrane potential on Na cotransports in eel intestinal brush-border membrane vesicles: Studies with a fluorescent dye. J. Membrain Biol. 101, 225–236 (1988). https://doi.org/10.1007/BF01872837

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  • DOI: https://doi.org/10.1007/BF01872837

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