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Thiol-dependent K∶Cl transport in sheep red cells: VIII. Activation through metabolically and chemically reversible oxidation by diamide

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Summary

The sulfhydryl (SH) oxidant diamide activated in a concentration-dependent manner ouabain-resistant (OR), Cl-dependent K flux in both low potassium (LK) and high potassium (HK) sheep red cells as determined from the rate of zero-trans K efflux into media with Cl or Cl replaced by NO3 or methane sulfonate (CH3SO3). Diamide did not alter the OR Na efflux into choline Cl. The diamide effect on K efflux appeared after 80% of cellular glutathione (GSH) was oxidized to GSSG, its disulfide. The stimulation of K efflux was completely reversed during metabolic restitution of GSH, a process that depended on the length of exposure to and the concentration of diamide. The action of diamide on both the K∶Cl transporter and GSH was also fully reversed by the reducing agent dithiothreitol (DTT). Diamide apparently oxidized the same SH groups alkylated by N-ethylmaleimide (NEM) (Lauf, P.K. 1983.J. Membrane Biol..73:237–246). Like NEM, diamide activated K∶Cl transport several-fold more in LK cells than in HK cells, and the effect on LK cells was partially inhibited by anti-L1, the allo-antibody known to inhibit OR K fluxes.

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  1. Department of Physiology and Biophysics, Wright State University, School of Medicine, 45401-0927, Dayton, Ohio

    P. K. Lauf

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Lauf, P.K. Thiol-dependent K∶Cl transport in sheep red cells: VIII. Activation through metabolically and chemically reversible oxidation by diamide. J. Membrain Biol. 101, 179–188 (1988). https://doi.org/10.1007/BF01872833

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  • DOI: https://doi.org/10.1007/BF01872833

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