Summary
Two-dimensional crystalline arrays of Ca2+-ATPase molecules develop after treatment of sarcoplasmic reticulum vesicles with Na3VO4 in a Ca2+-free medium. The influence of membrane potential upon the rate of crystallization was studied by ion substitution using oxonol VI and 3,3′-diethyl-2,2′-thiadicarbocyanine (Di−S−C2(5)) to monitor inside positive or inside negative membrane, potentials, respectively. Positive transmembrane potential accelerates the rate of crystallization of Ca2+-ATPase, while negative potential disrupts preformed Ca2+-ATPase crystals, suggesting an influence of transmembrane potential upon the conformation of Ca2+-ATPase.
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Beeler, T.J., Dux, L. & Martonosi, A.N. Effect of Na3VO4 and membrane potential on the structure of sarcoplasmic reticulum membrane. J. Membrain Biol. 78, 73–79 (1984). https://doi.org/10.1007/BF01872534
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DOI: https://doi.org/10.1007/BF01872534