Summary
The relationship between active Na transport (estimated by the short-circuit (SCC)) and active inorganic phosphate (Pi) transport was studied in the toad bladder. When SCC was inhibited by amiloride, ouabaim, or removal of K from the serosal bathing solution, active Pi transport was totally inhibited. When Na was replaced isotonically by choline in either the mucosal bathing solution or both the mucosal and serosal bathing solutions, there was no measurable SCC or active Pi transport. These experiments are compatible with the hypothesis that active Pi transport occurs only in the presence of active Na transport.
Similar content being viewed by others
References
Baumann, K., deRouffignac, C., Roinel, N., Rumrich, G., Ullrich, K.J. 1975. Renal phosphate transport: Inhomogeneity of local proximal transport rates and sodium dependence.Pfluengers Arch. 356:287
Hoffmann, N., Thees, M., Kinne, R. 1976. Phosphate transport by isolated renal brush border vesicles.Pfluegers Arch. 362:147
Kupfer, S., Kosovsky, J.D. 1970. Renal intracellular phosphate and phosphate excretion.Mt. Sinai J. Med. N.Y. 37:359
Sellers, B.B., Hall, J.A., Booth, C.W., Mendoza, S.A. 1977. Active phosphate transport across the urinary bladder of the toad,Bufo marinus.J. Membrane Biol. 32:291
Ullrich, K.J., Capasso, G., Rumrich, G., Papavassiliou, F., Kloss, S. 1977. Coupling between proximal tubular transport processes.Pfluegers Arch. 368:245
Ussing, H.H., Zerahn, K. 1951. Active transport of sodium as the source of electric current in the short-circuited frog skin.Acta Physiol. Scand. 23:110
Wen, S.F. 1974. Micropuncture studies of phosphate transport in the proximal tubule of the dog.J. Clin. Invest. 53:143
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sellers, B.B., Hall, J.A. & Mendoza, S.A. Effect of sodium transport inhibition on active phosphate transport by toad bladder. J. Membrain Biol. 41, 323–328 (1978). https://doi.org/10.1007/BF01871996
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01871996