The Journal of Membrane Biology

, Volume 75, Issue 3, pp 219–224 | Cite as

Rb+ influxes differentiate between growth arrest of cells by different agents

  • Rivka Panet
  • Ilana Fromer
  • Aviva Alayoff
Articles

Summary

The effect of cell cycle on Rb+ (K+) fluxes was studied in NIH 3T3 mouse fibroblasts. Serum starvation or isoleucine deprivation resulted in cell arrest at an earlyG1/G0 phase, accompanied by a marked decrease in both ouabainsensitive and ouabain-resistant Rb+ influx. On the other hand, cells arrested at lateG1/G0 phase by hydroxyurea treatment have high ouabain-sensitive and ouabain-resistant Rb+ influx. Butyric acid treatment resulted in cell arrest at an earlyG1/G0 phase, but in contrast to serum or isoleucine starvation did not decrease Rb+ influxes. It is thus shown that quiescent cells may have Rb+ influx rates as high as that of logarithmically growing cells. The results are consistent with the hypothesis that an increased ion permeability of the cell is initiated at a critical stage inG1/G0 phase, and that butyric acid may arrest the cell beyond that stage.

Key Words

ouabain-resistant ouabain-sensitive Rb+ influx 

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References

  1. Bakker-Grunwald, T. 1978. Effect of anions on potassium selfexchange in ascites tumor cells.Biochim. Biophys. Acta 513:292–295PubMedGoogle Scholar
  2. Bakker-Grunwald, T., Andrew, J.S., Neville, M.C. 1980. K+ influx components in ascites cells: The effects of agents interacting with the (Na++K+)-pump.J. Membrane Biol. 52:141–146CrossRefGoogle Scholar
  3. Burstin, S.J., Meiss, H.K., Basilico, C. 1974. A temperaturesensitive cell cycle mutant of the BHK cell line.J. Cell. Physiol. 84:397–408CrossRefPubMedGoogle Scholar
  4. D'Anna, J.A., Gurley, L.R., Becker, R.R., Barham, S.S., Tobey, R.A., Walters, R.A. 1980. Amino acid analysis and cell cycle dependent phosphorylation of an H1-like, buty-rate-enhanced protein (BEP; H10; IP25) from chinese hamster cells.Biochemistry 19:4331–4341CrossRefPubMedGoogle Scholar
  5. Geck, P., Pietrzyk, C., Burckhardt, B.-C., Pfeiffer, B., Heinz, E. 1980. Electrically silent cotransport of Na+, K+ and Cl in Ehrlich cell.Biochim. Biophys. Acta 600:432–447PubMedGoogle Scholar
  6. Jainchill, J.L., Aaronson, S.A., Todaro, G.J. 1969. Murine sarcoma and leukemia viruses: Assay using clonal lines of contact-inhibited mouse cells.J. Virol 4:549–553PubMedGoogle Scholar
  7. Kock, K.S., Leffert, H.L. 1979. Increased sodium ion influx is necessary to initiate rate hepatocyte proliferation.Cell 18:153–163PubMedGoogle Scholar
  8. Kohn, A. 1975. Differential effects of isoleucine deprivation on cell motility, membrane transport and DNA synthesis in Nill 8 hamster cells.Exp. Cell Res. 94:15–22CrossRefPubMedGoogle Scholar
  9. Kruh, J. 1982. Effects of sodium butyrate, a new pharmacological agent on cells in culture.Molec. Cell Biochem. 42:65–82PubMedGoogle Scholar
  10. Lauf, P.K., Valet, G. 1980. Cation transport in different volume population of genetically low K+ lamb red cells.J. Cell. Physiol. 104:283–293PubMedGoogle Scholar
  11. Martin, R.G., Stein, S. 1976. Resting state in normal and simian virus 40 transformed chinese hamster lung cells.Proc. Natl. Acad. Sci. USA 73:1655–1659PubMedGoogle Scholar
  12. Panet, R., Atlan, H. 1980. Characterization of a potassium carrier in rabbit reticulocyte cell membrane.J. Membrane Biol. 52:273–280Google Scholar
  13. Panet, R., Fromer, I., Atlan, H. 1982. Differentiation between serum stimulation of ouabain-resistant and sensitive Rb influx in quiescent NIH 3T3 cells.J. Membrane Biol. 70:165–169Google Scholar
  14. Pardee, A.B. 1974. A restriction point for control of normal animal cell proliferation.Proc. Natl. Acad. Sci. USA 71:1286–1290PubMedGoogle Scholar
  15. Rozengurt, E. 1980. Stimulation of DNA synthesis in quiescent cultured cells, exogenous agents, internal signals and early events.Curr. Top., Cell. Reg. 17:59–88Google Scholar
  16. Rozengurt, E., Heppel, L.A. 1975. Serum rapidly stimulates ouabain-sensitive86Rb+ influx in quiescent 3T3 cells.Proc. Natl. Acad. Sci. USA 72:4492–4495PubMedGoogle Scholar
  17. Smith, G.L. 1977. Increased ouabain-sensitive86rubidium uptake after mitogenic stimulation of quiescent chicken embryo fibroblasts with purified multiplication-stimulating activity.J. Cell. Biol. 73:761–767PubMedGoogle Scholar
  18. Smith, J.B., Rozengurt, E. 1978a. Serum stimulates the Na+, K+ pump in quiescent fibroblasts by increasing Na+ entry.Proc Natl. Acad. Sci USA 75:5560–5564PubMedGoogle Scholar
  19. Smith, J.B., Rozengurt, E. 1978b. Lithium transport by fibroblastic cells: Characterization and stimulation, by serum and growth factors in quiescent cultures.J. Cell Physiol. 97:441–450PubMedGoogle Scholar
  20. Tupper, J.T., Zorgniotti, F., Mills, B. 1977. Potassium transport and content duringG 1 andS phase following serum stimulation of 3T3 cells.J. Cell. Physiol. 91:429–440PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Rivka Panet
    • 1
  • Ilana Fromer
    • 1
  • Aviva Alayoff
    • 1
  1. 1.Department of Medical Biophysics and Nuclear MedicineHadassah Medical OrganizationJerusalemIsrael

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