The Journal of Membrane Biology

, Volume 107, Issue 3, pp 229–235 | Cite as

Quantitative analysis of outward rectifying K+ channel currents in guard cell protoplasts fromVicia faba

  • Julian I. Schroeder
Articles

Summary

A quantitative analysis of the time and voltage dependence of outward-rectifying K+ currents (\(I_{K^ + .out} \)) in guard cells fromVicia faba is described using the whole-cell patch-clamp technique. After step depolarizations from −75 mV to potentials positive to −40 mV, time-dependent outward currents were produced, which have recently been identified as K+ channel currents. This K+ current was characterized according to its time dependence and its steady-state activation.\(I_{K^ + .out} \) could be described in terms of a Hodgkin-Huxley type conductance. Activation of the current in time was sigmoid and was well fitted by raising the activation variable to the second power. Deactivating tail currents were single exponentials, which suggests that only one conductance underlies this slow outward K+ current. Rates of channel closing were strongly dependent on the membrane potential, while rates of channel opening showed only limited voltage dependence leading to a highly asymmetric voltage dependence for channel closing and opening. The presented analysis provides a quantitative basis for the understanding of\(I_{K^ + .out} \) channel gating and\(I_{K^ + .out} \) channel functions in plant cells.

Key Words

stomata ion channel rectifier Hodgkin-Huxley action potential repolarization patch clamp 

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Copyright information

© Springer-Verlag New York Inc. 1989

Authors and Affiliations

  • Julian I. Schroeder
    • 1
    • 2
  1. 1.Department of Membrane BiophysicsMax-Planck-Institut für biophysikalische ChemieGöttingenFederal Republic of Germany
  2. 2.Department of Physiology, Jerry Lewis Research Center, UCLA School of MedicineUniversity of California at Los AngelesLos Angeles

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