Skip to main content
Log in

Single Cl channels in molluscan neurones: Multiplicity of the conductance states

  • Articles
  • Published:
The Journal of Membrane Biology Aims and scope Submit manuscript

Summary

Properties of the single Cl channels were studied in excised patches of surface membrane from molluscan neurones using single-channel recording technique. These channels are controlled by Ca2+ and K+ acting on cytoplasmic and outer membrane surfaces, respectively, and by the membrane potential. The channels display about 16 intermediate conductance sublevels, each of them being multiples of ∼12.5 pS. The upper level of the channel conductance is about 200 pS. The channel behavior is consistent with an aggregation of channel-forming subunits into a cluster.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Barish, M.E. 1983. A transient calcium-dependent chloride current in the immatureXenopus oocyte.J. Physiol. (London) 342:309–325

    Google Scholar 

  • Barrett, J.N., Magleby, K.L., Pallotta, B.S. 1982. Properties of single calcium-activated potassium channels in cultured rat muscles.J. Physiol. (London) 331:211–230

    Google Scholar 

  • Benham, C.D., Bolton, T.B. 1983. Patch-clamp studies of slow potential-sensitive potassium channels in longitudinal smooth muscle cells of rabbit jejunum.J. Physiol. (London) 340:469–486

    Google Scholar 

  • Blatz, A.L., Magleby, K.L. 1983. Single voltage-dependent chloride-sensitive channels of large conductance in cultured rat muscle.Biophys. J. 43:237–241

    Google Scholar 

  • Cavalié, A., Ochi, R., Pelzer, D., Trautwein, W. 1983. Elementary currents through Ca2+ channels in guinea pig myocytes.Pfluegers Arch. 398:284–297

    Google Scholar 

  • Chemeris, N.K., Kazachenko, V.N., Kislov, A.N., Kurchikov, A.L. 1982. Inhibition of acetylcholine responses by intracellular calcium inLymnaea stagnalis neurones.J. Physiol. (London) 323:1–19

    Google Scholar 

  • Colquhoun, D., Neher, E., Reuter, H., Stevens, C.F. 1981. Inward current channels activated by intracellular Ca in cultured muscle cells.Nature (London) 294:752–754

    Article  Google Scholar 

  • Colquhoun, D., Sackmann, B. 1981. Fluctuations in microsecond range of the current through single acetylcholine receptor ion channels.Nature (London) 294:464–466

    Google Scholar 

  • Fenwick, E.M., Marty, A., Neher, E. 1982. Sodium and calcium channels in bovine chromaffin cells.J. Physiol. (London) 331:599–635

    Google Scholar 

  • Geletyuk, V.I., Kazachenko, V.N. 1983a. Single K+-dependent Cl channel in the molluscan neurones: Multiplicity of the conductance states.Dokl. Acad. Nauk SSSR 268:1245–1247 (in Russian)

    Google Scholar 

  • Geletyuk, V.I., Kazachenko, V.N. 1983b. Discreteness of the conductance of single potential-dependent K+ channel in the molluscan neurones.Biofizika 28:994–998 (in Russian)

    PubMed  Google Scholar 

  • Hamill, O.P., Bormann, J., Sakmann, B. 1983. Activation of multiple-conductance state chloride channels in spinal neurones by glycine and GABA.Nature (London) 305:805–808

    Google Scholar 

  • Hamill, O.P., Marty, A., Neher, E., Sakmann, B., Sigworth, F.J. 1981. Improved patch-clamp technique for high-resolution current recording from cells and cell-free membrane patches.Pfluegers Arch. 391:85–100

    Article  Google Scholar 

  • Hamill, O.P., Sakmann, B., 1981. Multiple conductance states of single acetylcholine receptor channels in embryonic muscle cells.Nature (London) 294:462–464

    Google Scholar 

  • Hutter, O.F., Warner, A.E. 1967. Action of some foreign cations and anions on the chloride permeability of frog muscle.J. Physiol. (London) 189:445–460

    Google Scholar 

  • Kazachenko, V.N., Geletyuk, V.I. 1983. Clustary organization of ionic channels.In: Biological Membranes and Function. (Abstr.) p. 183. III Soviet-Swiss Symposium. USSR Academy of Sciences, M.M. Shemyakin Institute of Bioorganic Chemistry. Tashkent

    Google Scholar 

  • Kazachenko, V.N., Geletyuk, V.I. 1984. The potential-dependent K+ channel in molluscan neurones is organized in a cluster of elementary channels.Biochim. Biophys. Acta 773:132–142

    PubMed  Google Scholar 

  • Kislov, A.N., Kazachenko, V.N. 1975. Potassium activation of the chloride conductance in the isolated snail neurones.Stud. Biophys. 48:151–153

    Google Scholar 

  • Kostenko, M.A., Geletyuk, V.I., Veprintsev, B.N. 1974. Completely isolated neurones in the molluscLymnaea stagnalis. A new objective for nerve cell biology investigation.Comp. Biochem. Physiol. 49A:89–100

    Google Scholar 

  • Krasts, I.V. 1978. The amplitude of action potential and calcium ion gradient on the membrane of mollusc neurone.Comp. Biochem. Physiol. 60A:195–197

    Google Scholar 

  • Lunevsky, V.Z., Zherelova, O.M., Vostrikov, I.Y., Berestovsky, G.N. 1983. Excitation ofCharaceae cell membrane as a result of activation of calcium and chloride channels.J. Membrane Biol. 72:43–58

    Google Scholar 

  • Manella, C.A., Colombini, M., Frank, J. 1983. Structural and functional evidence for multiple channel complexes in the outer membrane ofNeurospora crassa mitochondria.Proc. Natl. Acad. Sci. USA 80:2243–2247

    PubMed  Google Scholar 

  • Sauvé, R., Roy, G., Payet, D. 1983. Single channel currents fromHela cells.J. Membrane Biol. 74:41–50

    Google Scholar 

  • Siegelbaum, S.A., Camarado, J.S., Kandel, E.R. 1982. Serotonin and cyclic AMP close single K+ channels inAplysia sensory neurones.Nature (London) 299:413–417

    Google Scholar 

  • Strickholm, A. 1981. Ionic permeability of K+, Na+ and Cl in potassium depolarized nerve.Biophys. J. 35:677–697

    PubMed  Google Scholar 

  • Thomas, S.H., Aldrich, R.W. 1980. Membrane potassium channels.In: The Cell Surface and Neuronal Function. C.W. Cotman, G. Poste, and G.L. Nicolson, editors. pp. 49–85. Elseveier/North-Holland Biomedical, Amsterdam

    Google Scholar 

  • Volkova, S.P., Lunevsky, V.Z., Spiridonov, N.A., Vinokurov, M.G., Berestovsky, G.N. 1980. About chemical composition of the Ca2+ channels inCharaceae cells.Biofizika 25:537–541 (in Russian)

    PubMed  Google Scholar 

  • White, M.M., Miller, C. 1979. A voltage-gated anion channel from the electric organ ofTorpedo californica.J. Biol. Chem. 254:10161–10166

    Google Scholar 

  • Yellen, G. 1982. Single Ca2+ activated nonselective cation channels in neuroblastoma.Nature (London) 296:357–359

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Geletyuk, V.I., Kazachenko, V.N. Single Cl channels in molluscan neurones: Multiplicity of the conductance states. J. Membrain Biol. 86, 9–15 (1985). https://doi.org/10.1007/BF01871605

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01871605

Key Words

Navigation