Summary
Properties of the single Cl− channels were studied in excised patches of surface membrane from molluscan neurones using single-channel recording technique. These channels are controlled by Ca2+ and K+ acting on cytoplasmic and outer membrane surfaces, respectively, and by the membrane potential. The channels display about 16 intermediate conductance sublevels, each of them being multiples of ∼12.5 pS. The upper level of the channel conductance is about 200 pS. The channel behavior is consistent with an aggregation of channel-forming subunits into a cluster.
Similar content being viewed by others
References
Barish, M.E. 1983. A transient calcium-dependent chloride current in the immatureXenopus oocyte.J. Physiol. (London) 342:309–325
Barrett, J.N., Magleby, K.L., Pallotta, B.S. 1982. Properties of single calcium-activated potassium channels in cultured rat muscles.J. Physiol. (London) 331:211–230
Benham, C.D., Bolton, T.B. 1983. Patch-clamp studies of slow potential-sensitive potassium channels in longitudinal smooth muscle cells of rabbit jejunum.J. Physiol. (London) 340:469–486
Blatz, A.L., Magleby, K.L. 1983. Single voltage-dependent chloride-sensitive channels of large conductance in cultured rat muscle.Biophys. J. 43:237–241
Cavalié, A., Ochi, R., Pelzer, D., Trautwein, W. 1983. Elementary currents through Ca2+ channels in guinea pig myocytes.Pfluegers Arch. 398:284–297
Chemeris, N.K., Kazachenko, V.N., Kislov, A.N., Kurchikov, A.L. 1982. Inhibition of acetylcholine responses by intracellular calcium inLymnaea stagnalis neurones.J. Physiol. (London) 323:1–19
Colquhoun, D., Neher, E., Reuter, H., Stevens, C.F. 1981. Inward current channels activated by intracellular Ca in cultured muscle cells.Nature (London) 294:752–754
Colquhoun, D., Sackmann, B. 1981. Fluctuations in microsecond range of the current through single acetylcholine receptor ion channels.Nature (London) 294:464–466
Fenwick, E.M., Marty, A., Neher, E. 1982. Sodium and calcium channels in bovine chromaffin cells.J. Physiol. (London) 331:599–635
Geletyuk, V.I., Kazachenko, V.N. 1983a. Single K+-dependent Cl− channel in the molluscan neurones: Multiplicity of the conductance states.Dokl. Acad. Nauk SSSR 268:1245–1247 (in Russian)
Geletyuk, V.I., Kazachenko, V.N. 1983b. Discreteness of the conductance of single potential-dependent K+ channel in the molluscan neurones.Biofizika 28:994–998 (in Russian)
Hamill, O.P., Bormann, J., Sakmann, B. 1983. Activation of multiple-conductance state chloride channels in spinal neurones by glycine and GABA.Nature (London) 305:805–808
Hamill, O.P., Marty, A., Neher, E., Sakmann, B., Sigworth, F.J. 1981. Improved patch-clamp technique for high-resolution current recording from cells and cell-free membrane patches.Pfluegers Arch. 391:85–100
Hamill, O.P., Sakmann, B., 1981. Multiple conductance states of single acetylcholine receptor channels in embryonic muscle cells.Nature (London) 294:462–464
Hutter, O.F., Warner, A.E. 1967. Action of some foreign cations and anions on the chloride permeability of frog muscle.J. Physiol. (London) 189:445–460
Kazachenko, V.N., Geletyuk, V.I. 1983. Clustary organization of ionic channels.In: Biological Membranes and Function. (Abstr.) p. 183. III Soviet-Swiss Symposium. USSR Academy of Sciences, M.M. Shemyakin Institute of Bioorganic Chemistry. Tashkent
Kazachenko, V.N., Geletyuk, V.I. 1984. The potential-dependent K+ channel in molluscan neurones is organized in a cluster of elementary channels.Biochim. Biophys. Acta 773:132–142
Kislov, A.N., Kazachenko, V.N. 1975. Potassium activation of the chloride conductance in the isolated snail neurones.Stud. Biophys. 48:151–153
Kostenko, M.A., Geletyuk, V.I., Veprintsev, B.N. 1974. Completely isolated neurones in the molluscLymnaea stagnalis. A new objective for nerve cell biology investigation.Comp. Biochem. Physiol. 49A:89–100
Krasts, I.V. 1978. The amplitude of action potential and calcium ion gradient on the membrane of mollusc neurone.Comp. Biochem. Physiol. 60A:195–197
Lunevsky, V.Z., Zherelova, O.M., Vostrikov, I.Y., Berestovsky, G.N. 1983. Excitation ofCharaceae cell membrane as a result of activation of calcium and chloride channels.J. Membrane Biol. 72:43–58
Manella, C.A., Colombini, M., Frank, J. 1983. Structural and functional evidence for multiple channel complexes in the outer membrane ofNeurospora crassa mitochondria.Proc. Natl. Acad. Sci. USA 80:2243–2247
Sauvé, R., Roy, G., Payet, D. 1983. Single channel currents fromHela cells.J. Membrane Biol. 74:41–50
Siegelbaum, S.A., Camarado, J.S., Kandel, E.R. 1982. Serotonin and cyclic AMP close single K+ channels inAplysia sensory neurones.Nature (London) 299:413–417
Strickholm, A. 1981. Ionic permeability of K+, Na+ and Cl− in potassium depolarized nerve.Biophys. J. 35:677–697
Thomas, S.H., Aldrich, R.W. 1980. Membrane potassium channels.In: The Cell Surface and Neuronal Function. C.W. Cotman, G. Poste, and G.L. Nicolson, editors. pp. 49–85. Elseveier/North-Holland Biomedical, Amsterdam
Volkova, S.P., Lunevsky, V.Z., Spiridonov, N.A., Vinokurov, M.G., Berestovsky, G.N. 1980. About chemical composition of the Ca2+ channels inCharaceae cells.Biofizika 25:537–541 (in Russian)
White, M.M., Miller, C. 1979. A voltage-gated anion channel from the electric organ ofTorpedo californica.J. Biol. Chem. 254:10161–10166
Yellen, G. 1982. Single Ca2+ activated nonselective cation channels in neuroblastoma.Nature (London) 296:357–359
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Geletyuk, V.I., Kazachenko, V.N. Single Cl− channels in molluscan neurones: Multiplicity of the conductance states. J. Membrain Biol. 86, 9–15 (1985). https://doi.org/10.1007/BF01871605
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01871605