Summary
The present study investigated whether the hydrophobic properties (wettability) of the luminal surface of the toad urinary bladder might play a role in modulating water transport across this epithelium. In the absence of vasopressin (ADH), water transport across the tissue was low, while luminal surface hydrophobicity (water contact angle) was relatively high. Following stimulation by ADH, water transport increased and surface hydrophobicity decreased. The addition of indomethacin to inhibit ADH-induced prostaglandin synthesis did not reduce these actions of ADH. In an attempt to alter water transport in this tissue, a liposomal suspension of surface-active phospholipids was administered to the luminal surface. This addition had no detectable influence on the low basal rates of water transport, but blocked the ADH-induced stimulation of water transport. We suggest that surface-active phospholipids on the toad bladder luminal membrane may contribute to the hydrophobic characteristics of this tissue. ADH may act to decrease surface hydrophobicity, facilitating the movement of water molecules across an otherwise impermeable epithelium. This surface alteration may be associated with the appearance of water channels in the apical membrane.
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Bar, H.-P., Hechter, O., Schwartz, I. L., Walter, R. 1970. Neurohypophyseal hormone-sensitive adenyl cyclase of toad urinary bladder.Proc. Nat. Acad. Sci. USA 67:7–12
Bentley, P. J. 1958. The effects of neurohypophysial extracts on water transfer across the wall of the isolated urinary bladder of the toadBufo marinus.Endocrinology 17:201–209
Billah, M. M., Michell, R. H. 1979. Phosphatidylinositol metabolism in rat hepatocytes stimulated by glycogenolytic hormones.Biochem. J. 182:661–668
Dibona, D. R. 1983. Cytoplasmic involvement of ADH-mediated omosis across toad urinary bladder.Am. J. Physiol. 245:C297-C307
Handler, J. S., Butcher, R. W., Sutherland, E. W., Orloff, J. 1965. The effect of vasopressin and of theophylline on the concentration of adenosine 3′,5′-phosphate in the urinary bladder of the toad.J. Biol. Chem. 240:4524–4526
Hays, R. M. 1983. Alteration of luminal membrane structure by antidiuretic hormone.Am. J. Physiol. 245:C289-C296
Hills, B. A. 1985. Gastric mucosal barrier: Stabilization of hydrophobic lining to the stomach by mucus.Am. J. Physiol. 249:G342-G349
Hills, B. A., Barrow, R. E. 1979. The contact angle induced by DPL at pulmonary epithelial surfaces.Respir. Physiol. 38:173–183
Hills, B. A., Butler, B. D., Lichtenberger, L. M. 1983. Gastric mucosal barrier: Hydrophobic lining to the lumen of the stomach.Am. J. Physiol. 244:G561-G568
Levine, S. D., Kachadorian, D. N., Schlondorff, D. 1981. Effects of trifluoperazine on function and structure of toad urinary bladder: Role of calmodulin in vasopressin-stimulation of water permeability.J. Clin. Invest. 676:662–672
Lichtenberger, L. M., Grazini, L. A., Dial, E. J., Butler, B. D., Hills, B. A. 1983. Role of surface-active phospholipids in gastric cytoprotection.Science 219:1327–1329
Lichtenberger, L. M., Richards, J. E., Hills, B. A. 1983. Effect of 16,16-dimethyl prostaglandin E2 on the surface hydrophobicity of aspirin-treated canine gastric mucosa.Gastroenterology 88:308–314
Lichtenberger, L. M., Romero, J. J. 1987. Gastric protective effect of unsaturated species of phosphatidylcholine: Dependence on cholesterol.Gastroenterology 92:1506 (abstr.)
Monaco, M. E. 1982. The phosphatidylinositol cycle in WRK-1 cells.J. Biol. Chem. 257:2137–2139
Orloff, J., Handler, J. S., Bergstrom, S. 1965. Effect of prostaglandin (PGE1) on the permeability response of toad bladder to vasopressin, theophylline and adenosine, 3′,5′-monophosphate.Nature (London) 205:397–398
Schlondorff, D., Franki, N. 1980. Effect of vasopressin on cyclic AMP-dependent protein kinase in toad urinary bladder.Biochim. Biophys. Acta 628:1–12
Wade, J. B. 1986. Role of membrane fusion in hormonal regulation of epithelial transport.Annu. Rev. Physiol. 48:213–223
Zusman, R. M., Keiser, H. R., Handler, J. S. 1977. Vasopressin-stimulated prostaglandin E biosynthesis in the toad urinary bladder: Effect on water flow.J. Clin. Invest. 60:1339–1347
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Dial, E.J., Huang, J., O'Neil, R.G. et al. Surface hydrophobicity and water transport of the toad urinary bladder: Effects of vasopressin. J. Membrain Biol. 106, 119–122 (1988). https://doi.org/10.1007/BF01871393
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DOI: https://doi.org/10.1007/BF01871393